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Seeing the unseen of Chinese herbal medicine processing (Paozhi): advances in new perspectives
Chinese Medicine volume 13, Article number: 4 (2018)
Processing (Paozhi) represents a unique Chinese pharmaceutic technique to facilitate the use of Chinese herbal medicines (CHMs) for a specific clinical need in the guidance of Traditional Chinese Medicine (TCM) theory. Traditionally, most CHMs require a proper processing to meet the needs of specific clinical syndromes before being prescribed by TCM practitioners. During processing, significant changes in chemical profiles occur, which inevitably influence the associated pharmacological properties of a CHM. However, although processing is formed in a long-term practice, the underlying mechanisms remain unclear for most CHMs. The deepening understanding of the mechanism of processing would provide scientific basis for standardization of processing. This review introduced the role of processing in TCM and several typical methods of processing. We also summarized the up-to-date efforts on the mechanistic study of CHM processing. The processing mechanisms mainly include the following aspects: (i) directly reducing contents of toxic constituents; (ii) structural transformation of constituents; (iii) improving solubility of constituents; (iv) physically changing the existing form of constituents; (v) and influence by excipients. These progress may give new insights into future researches.
Processing, Paozhi in Chinese, is an ancient Chinese pharmaceutic technique to facilitate the use of Chinese herbal medicines (CHMs) for a specific clinical need in the guidance of Traditional Chinese Medicine (TCM) theory . Processing of CHMs develops along with the history of TCM and promotes the formation of TCM theory in long-term practice, even wine serves as part of the ancient Chinese character ‘medicine’ for all its important role. Most CHMs need to be elaborately processed to become decoction pieces prior to their final consumption in the clinic or manufacture of proprietary drugs . Processing represents a unique Chinese pharmaceutic approach that differentiates CHMs from other medicinal herbs in the world. In Chinese Pharmacopoeia (CP, 2015 edition), decoction piece(s) and related processing method(s) are clearly listed as a specific item of a CHM, and some decoction pieces like Astragali Radix Preparata Cum Melle are recorded as a separate CHM with independent quality control standards and indications . In contrast, only few processed medicinal herbs and processing methods are recorded in the pharmacopoeias of other countries .
Processing encompasses a series of techniques such as cutting, crushing, roasting, baking, and stir-frying with or without liquid/solid excipient, by which decoction pieces with different therapeutic potency can be derived from the same herb . For instance, Pinelliae Rhizoma (PR) is a commonly used CHM for the treatment of phlegm-induced cough, vomit and headache . Four processed PR are recorded in the latest CP, namely raw PR, PR Praeparatum (PRP, processed with 15% Glycyrrhizae Radix et Rhizoma and 10% lime), PR Praeparatum cum Zingibere et Alumine (PRZA, processed with 25% Zingiberis Rhizoma Recens and 12.5% alume) and PR Praeparatum cum Alumine (PRPA, processed with 20% alume) . These decoction pieces produced by different processing methods are developed to reduce the toxicity of PR  and to guide and concentrate its therapeutic effects. Raw PR is often externally used for treatment of carbuncle and furuncle, PRP is inclined to relieve phlegm-caused cough, dizziness and headache, while PRZA and PRPA are respectively prescribed for phlegm-caused vomit and cough (Fig. 1).
Generally, processing can reduce toxicity, reinforce efficacy, alter energetic nature and therapeutic direction, as well as improve flavor of CHMs, thereby increase the therapeutic effectiveness and applicability of CHMs in individualized treatment. However, despite the extensive use of processed CHM, the underlying mechanisms of processing remain unclear for most CHMs to date. During the processing, particularly under heating and/or moist conditions, complicated changes in herbal components of CHMs may occur: the contents be increased or decreased; structures be changed; and/or novel compounds be formed. In many cases, the contents and structures of constituents may be altered simultaneously. Along with these changes mediated by processing, the pharmacological activity of a certain CHM may be changed accordingly. Therefore, investigation of the chemical and pharmacological changes of CHM before and after processing is key for understanding of underlying mechanisms. In the past few decades, emerging studies have been carried out to elucidate the mechanisms of processing. Herein, this review summarizes the up-to-date knowledge on these aspects, aiming to provide new insights to future researches.
Methods of processing
The first recordation of processing can be dated back to 200 BC in Recipes for 52 Ailments (Wushi’er Bingfang), in which some classical methods like burning, calcining, stewing, and soaking were listed . In the Northern and Southern dynasties, Master Lei’s discourse on processing (Leigong Paozhi Lun) appeared as the earliest book that systemically described the principles and methods of processing . Afterwards, there are a series of monographs of processing that record and summarize the experiences of TCM practitioners. In broad terms, processing describes every procedure involved in preparing raw plants (or animal or mineral) into decoction pieces. In this review we mainly discuss these specific methods applied when the CHMs are cleaned, cut, and dried. Some commonly used processing methods are described below and listed in Table 1.
Cleaned and cut crude CHMs are fried in a pot, with or without the aid of excipients, while being constantly stirred until a certain degree of frying is obtained.
Stir-frying without excipients
Usually there are three degrees of stir-frying evaluated by the color in appearance and/or odor of a specific herb: stir-frying till yellow, till charred, and till carbonized (black outside and charred inside). Crataegi Fructus is a typical CHM that can be stir-fried until different degrees for distinct therapeutic purpose . Un-processed Crataegi Fructus can promote digestion and invigorate blood circulation while stir-fried Crataegi Fructus is mainly used for indigestion. In contrast, charred Crataegi Fructus and carbonized Crataegi Fructus are used for the treatment of indigestion-caused diarrhea and gastrointestinal hemorrhage, respectively.
Stir-frying with liquid excipients
In order to reinforce and/or guide the efficiency of the herbs, many liquid excipients like yellow rice wine, vinegar and honey are often added to the crude herbs prior to stir-frying. For instance, processing with wine can enhance the effect of Angelicae Sinensis Radix in invigorating blood circulation , and wine-fried Angelicae Sinensis Radix is widely prescribed in many famous TCM formulae including Danggui Buxue decection, Siwu Decoction and Longdan Xiegan Pills.
Stir-frying with solid excipients
Similar to liquid excipient-assisted stir-frying, stir-frying with solid excipients also helps to extend the utility of CHMs. Stir-frying with rice represents an important approach of TCM practitioners to reduce the toxicity of some poisonous CHMs such as Mylabris  and reinforce the effect of many spleen-tonifying CHMs including Codonopsis Radix .
Steaming is a commonly used processing method to alter the properties of various CHMs by steaming the crude herbs with or without additional excipients. For example, steaming raw Polygoni Multiflori Radix with black bean juice can turn the anti-malarial and defecating effects to tonifying effects like liver and kidney replenishing, hair blackening, and bone strengthening [13, 14].
Boiling CHMs in water or in a herbal decoction can also (i) minimize the side effect of CHMs, such as Glycyrrhizae Radix decoction boiled Polygalae Radix to reduce the irritation on throat ; or (ii) enhance the therapeutic effect, such as vinegar boiled Curcumae Rhizoma to reinforce the effect in removing blood stasis.
Wrapping CHMs in moistened papers, bran or mud, and heating until the envelop becomes cracked or charred is another approach to reduce the undesired constituents and reinforce the astringent effect of CHMs. Wheat bran-stewed Myristicae Semen is the major form of Myristicae Semen in clinical application due to reduced irritant oils . Stewing using moistened straw paper endows Aucklandiae Radix with stronger astringent property and enhance the anti-diarrhea effect .
Other processing methods
Many other methods are widely applied to guarantee the safety and effectiveness of CHMs. For instance, water trituration is a repetitious and complicated process by grounding mineral CHMs with water to obtain extremely fine powder. Many mineral and crustaceous CHMs can be calcined directly or indirectly in the flames to render these hard CHMs crisp and thus easy to crush.
Advances in understanding the mechanism of processing
Processing is an important feature of CHM, which is formed early in the history of TCM and has developed along with its clinical practice. The methods and purposes of processing are usually different for different herbs, while processing might have multiple influences on a certain herb. In TCM theory, disease is often a result of imbalance between Yin and Yang in human body. It is believed that processing can adjust the nature (heat, warm, cold and cool) of a certain CHM to facilitate the symptomatic and accurate prescription by TCM practitioners and help equilibrate the balance between Yin and Yang in human body. In this regard, traditionally, most CHMs require proper processing before being prescribed. Processing may directly reduce the contents of toxic constituents, transform the structure of constituents, or increase the solubility of active constituents (Fig. 2). Efforts have been made in recent years to understand the traditional aspect of processing. Some representative evidences in elucidating the mechanisms of CHM processing are displayed in Table 2.
Directly reducing contents of toxic constituents
The primary concept of detoxification is to reduce the contents of toxic constituents in CHM. Processing has been proved as a useful means to reduce the toxicity of certain CHMs. Toxic compounds usually possess unique physical characteristics. Based on this, specific processing methods may efficiently reduce their contents in the corresponding CHMs.
Mylabris (Banmao), is derived from the blister beetles Mylabris phalerata Pallas or M. cichorii Linnaeus, and is a famous poisonous CHM using for treating cancers [59, 60]. The internal use of Banmao often leads to serious nephrotoxicity which is lethal . Traditionally, Mylabris is stir-frying processed with or without the presence of rice. In recent years, Mylabris is also processed with sodium hydroxide solutions. Both methods have been proved to reduce its toxicity . It has been demonstrated that cantharidin, a terpenoid defensive toxin, is responsible for the therapeutic action as well as toxicity of Mylabris [63,64,65]. Therefore, control of the contents of cantharidin is key for safe and effective use of Mylabris. A number of studies show that cantharidin can be readily sublimated when the processing temperature reaches 120 °C, and thus its contents in raw materials are significantly reduced . Furthermore, in alkaline condition of sodium hydroxide solution, cantharidin becomes the form of cantharidinate sodium, which is less nephrotoxic than the original form [67, 68]. Based on these findings, different processing methods result in the decreased contents of highly-toxic cantharidin and thus reduce the toxicity of Mylabris.
Crotonis Semen (Badou, in Chinese) is the dried fruit of Croton tiglium L., and is used in TCM for treatment of ascites, constipation, diphtheritis, acute laryngitis and laryngeal obstruction . Raw Crotonis Semen is highly toxic and can cause hemolysis and severe diarrhea. It is demonstrated that the toxic components mainly exist in the Croton oil [70, 71]. Traditional processing method to remove oil from Crotonis Semen can remarkably reduce the contents of toxic constituents, resulting in reduced toxicity.
Structural transformation of constituents
Many methods of processing, such as stir-frying, steaming and boiling, necessitate the heating and/or moist conditions, which inevitably leads to complex chemical changes in processed CHMs. Structural transformation of herbal components is one of the most common consequences due to processing. Herbal components may undergo oxidation, decomposition, isomerization, hydrolysis and/or reaction with other constituents, eventually, to form novel compounds . This often results in alteration of pharmacological or toxicological properties of processed CHMs compared to the raw ones. Some of CHMs, including the Aconitum root, Ginseng Radix et Rhizome and Rhei Radix et Rhizoma, have been demonstrated to possess distinct chemical profiles after processing and show reduced toxicity or altered therapeutic activities.
Aconitum root: decomposing of highly toxic components during processing leads to detoxification
Chuanwu (Aconiti Radix, the mother root of A. carmichaeli), Fuzi (A. Lateralis Radix, the daughter root of A. carmichaeli) and Caowu (A. kusnezoffii Radix, the root of A. kusnezoffii) are three most popular Aconitum herbs used in TCM and are documented in the latest CP [73, 74]. Raw Aconitum plants are extremely dangerous, and can only applied in external use. They are used in decoction, proprietary medicines and other formulations only after being properly processed (repeated boiling or steaming). Aconitum root induces remarkable cardiotoxicity and neurotoxicity. The toxidrome of acute aconite poisoning is a combination of cardiovascular, neurological, gastrointestinal and other symptoms . Despite their toxicity and narrow therapeutic window, Aconitum root has been widely used in TCM due to their anti-inflammatory, analgesic and cardiotonic properties . Till now, there are six different types of processed Aconitum medicinals, including Zhichuanwu, Yanfuzi, Danfupian, Heishunpian, Baifupian and Zhicaowu, which are documented in the latest CP. Regardless of the distinct processing methods, many researches have demonstrated that properly processed Aconitum root showed reduced toxicity [77, 78].
The toxicity of Aconitum herbs is mainly due to the presence of Aconitum alkaloids at high concentrations [79, 80]. These alkaloids have been found to target voltage-sensitive sodium channels in myocardium, nerves and muscles, and cause cardiotoxicity and neurotoxicity [81, 82]. C19-diterpenoid-type alkaloids are found to be the main constituents of aconitum . These alkaloids are further classified into four types: diester diterpenoid alkaloids (DDA), such as aconitine, mesaconitine, and hypaconitine; monoester diterpenoid alkaloids (MDA), such as benzoylaconine, benzoylhypaconine, and benzoylmesaconine; non-ester diterpenoid alkaloids (NDA), such as aconine, mesaconine, and hypaconine; and lipoalkaloids. A series of studies have demonstrated that the DDA can be decomposed into MDA by losing an acetic acid at C-8 position during processing, which further undergo elimination of a benzoylic acid at C-14 position to generate NDA, or substitution with a fatty acid acyl group at C-8 position to form lipoalkaloids [18,19,20]. For instance, at the heating and moist condition (boiling or steaming), aconitine, mesaconitine and hypaconitine could be firstly converted into benzoylaconine, benzoylmesaconine and benzoylhypaconine, respectively, and further transformed into aconine, mesaconine, and hypaconine, respectively [83, 84]. After processing, the contents of the DDA (aconitine, mesaconitine and hypaconitine) were significantly reduced in Fuzi . Since DDA are much toxic (100- to 400-fold) than MDA and lipoalkaloids, decomposing of DDA has been identified as the main mechanism for detoxification of aconitum processing . Notably, MDA and lipoalkaloids also display remarkable anti-inflammatory and analgesic effects.
Traditionally, the processing of Aconitum root is monitored by tasting the spicy flavor which should gradually fade to certain extent. With the understanding of the underlying mechanisms, processing of aconitum is now controlled by determination of the marker alkaloids. For instance, as recorded in the latest CP, the total contents of DDA-type constituents should not be higher than 0.02% (g/g), while the contents of NDA-type constituents should be no less than 0.01% (g/g).
Ginseng: structural transformation of ginsenosides during processing results in enhanced efficacy
Ginseng Radix et Rhizome (Renshen, in Chinese) has been traditionally used in TCM for thousands of years, and is also one of the most popular functional food in Asian countries [85, 86]. Ginsenosides, the triterpene saponins, have been found to be the main bioactive constituents in ginseng, which are responsible for anti-oxidant, antidiabetic, immune modulatory, anti-inflammatory and anti-cancer properties [87,88,89]. Their structures are mainly grouped into dammarane type with 20(S)-protopanaxadiol and 20(S)-protopanaxatriol as the aglycone and oleanane type .
White ginseng (the fresh ginseng air-dried) and the processed one, Hongshen (the fresh ginseng steamed for 2–3 h and dried), are two types of ginseng products available in the market. Traditionally, Hongshen is considered to be more powerful in “boosting yang” than the White ginseng [91, 92]. Several reports have suggested that certain activities of Hongshen are better than the White ginseng . During processing (steaming), complex chemical changes occur in terms of ginsenosides. The malonyl-ginsenosides, which are only found in the white ginseng, are de-malonylated and converted into the corresponding ginsenosides [94, 95]. The sugar chains at C-20 and/or C-3 are further hydrolyzed . Furthermore, the 20(S)-type ginsenosides can be transformed into 20(R)-type [90, 94, 95]. As a result, the chemical profile of White ginseng and Hongshen are considerably different. The polar ginsenosides in White ginseng becomes the less polar ones. The characteristic ginsenosides in Hongshen include 20(S)-, 20(R)-Rg3, Rk3, Rh4, Rk1, Rg5, etc., which have been demonstrated to exhibit more potent anti-cancer, anti-diabetic, and anti-inflammatory effects [96, 97]. Therefore, structural transformation of ginsenosides during processing results in enhanced efficacy of the steamed ginseng.
Improved solubility of active constituents
Emerging evidences indicate that processing improves the solubility of herbal constituents in certain CHMs. Under heating condition, excipients used in processing such as wine and vinegar often help active constituents more easily to dissolve from a complex texture. Eventually, the processed CHMs show enhanced efficacy.
Coptidis Rhizoma (Huanglian, in Chinese) is derived from the dried rhizome of Coptis chinensis Franch., C. deltoidea C. Y. Cheng et Hsiao or C. teeta Wall, and is traditionally used for toothache, dysentery, hypertension, inflammation and liver diseases [98, 99]. Alkaloids, such as berberine, palmatine, epiberberine and coptisine, are found to be one of the main types of active constituents . It is reported that the dissolution rate of total alkaloids in wine-processed Coptidis Rhizoma reaches 90%, while that in raw medicinals is only 58%. After processing, the contents of berberine, palmatine, coptisine and jatrorrhizine that were detected in the processed Coptidis Rhizoma were significantly increased . This observation is also seen on Angelicae Sinensis Radix (Danggui, in Chinese). Danggui, the dried root of Angelica sinensis (Oliv.) Diels., is a famous CHM and has been used for more than 2000 years in China as a dietary supplement for women’s health . A recent study showed that yellow wine-processed Danggui displays a significant increase in solubility of ferulic acid, one of the major biological components .
Physically changing the existing form of constituents
Processing can also change the existing form of constituents in CHMs, which may influence their actions. One example is the PR, the dried tuber of P. ternata (unb.) Breit. It is first recorded in Shen-Nong-Ben-Cao-Jing (Shen Nong’s Herbal Classic, B.C. 100–200), and is widely used in TCM to treat cough, phlegm, vomiting and cancer [25, 101]. Similar to the Aconitum, raw PR is very toxic and can be only applied for external use. In order to reduce its toxicity, alum solution is always used in the processing of PR. Recent studies showed that aluminium ions in the alum solution were capable of complexing with oxalic acid in calcium oxalate of raphides, which helped to dissolve calcium oxalate and thus altered the unique rigid crystal structure . This further led to the dissolve and degradation of the lectin inside the raphides . As a result, the pro-inflammatory effect of raphides was significantly decreased. Therefore, physically structural alteration of needle-like calcium oxalate crystals contributes to the reduction of toxicity of PR during processing.
Influences of excipients
Excipients, including wine, vinegar, ginger juice, honey, rice, Glycyrrhizae Radix et Rhizoma, Euodiae Fructus and mutton fat, are frequently used in processing of CHMs to meet different purposes, and sometimes play an important role. Wine, vinegar and honey are commonly used as solvents to promote the solubility of several types of naturally-occurring compounds. As discussed above, wine can help the dissolve of active constituents of Danggui and Huanglian [10, 57]. Meanwhile, some excipients can react with the constituents in specific CHMs. For instance, during vinegar-assisted processing the toxic diterpenes in Kansui Radix (Gansui) can react with acetic acid to form acetylated diterpenes with poor solubility, which results in reduced toxicity [51, 52].
Notably, some excipients themselves, such as Glycyrrhizae Radix et Rhizoma, Euodiae Fructus and honey, are derived from CHMs and have their own therapeutic effects. Several studies show that constituents from these excipients are important for reducing toxicity and/or enhancing efficacy. As above described, 25% juice of Zingiberis Rhizoma Recens is used in the processing of PR Praeparatum cum Zingibere et Alumine (Jiangbanxia). It is demonstrated that gingerol derived from the ginger juice can remarkably inhibit Banxia-induced inflammation, which contributes to the detoxification effect . Euodiae Fructus (Wuzhuyu) is the dried fruit of E. rutaecarpa (Juss.) Benth., E. rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang, or E. rutaecarpa (Juss.) Benth. var. bodinieri (Dode) Huang, and its processed products are produced by boiling raw materials with Glycyrrhizae Radix [103, 104]. Studies have shown that Glycyrrhizae Radix can enhance the analgesic effects of Wuzhuyu. After processing, the content of hydroxyevodiamine is reduced significantly, while that of evocarpine is increased .
Conclusion and future perspectives
Processing is formed in long-term practice with a systematic theory, and represents one of the therapeutic wisdoms of TCM. Since most crude materials of CHMs require proper processing before being used, standardization of processing is a prerequisite for standardization of CHM. However, it is of much difficulty in terms of this aspect. Firstly, the methods of processing vary significantly in different regions of China . For certain CHMs, there is no unified processing practice for all areas of China. Although there are a total of 618 decoction pieces that have been adopted in the latest CP, a large number of processed CHMs are not covered. Most CHMs recorded in the local standards of different provinces have used different methods . The use of excipients also sometimes varies . Secondly, even in the latest CP, the processing practice is not accurately described. It is reported that the bioactive or toxic constituents can be changed over time and processing temperature [107,108,109]. The use of excipients is also important. For instance, different types and concentration of wine have distinct impact on the main compositions and contents of the alkaloids of Coptis chinensis . Notably, there is no standards for most excipients used. Based on these facts, it is difficult to control the procedure of processing in practice. Traditionally, pharmaceutical workers process CHMs mainly according to their experiences to judge the color, flavor or appearance of CHMs. In a recent study, Fei et al. analyzed the color values of the peel and flesh of Crataegi Fructus and constructed related mathematical functions to effectively evaluate the processing degree of Crataegi Fructus . Some researchers have also suggested to use novel techniques such as microwaves, which can be easily controlled [111, 112]. However, whether these new evaluation systems or techniques are able to produce qualified products still needs more assessment before applying to industry. Till now, the efforts for optimization and standardization of processing are still largely needed.
Another challenge is the standardization of decoction pieces, especially the processed CHMs. At current stage, there are no quality control standards for most processed CHMs. As described in this review, there are complex chemical changes in processing which are usually associated with alterations in pharmacological effects. Therefore, the deepening understanding of the underlying mechanisms of processing is of great significance for the standardization of CHMs including the selection of markers.
Investigation of the mechanisms of processing has been ongoing for several decades. With the development of novel concepts, techniques and models, great advances have been achieved, although most parts of processing remain unclear. In this review, we have summarized current progress with regards of processing mechanisms into the following aspects: (i) directly reducing contents of toxic constituents; (ii) structural transformation of constituents; (iii) improving solubility of constituents; (iv) physically changing the existing form of constituents; (v) influence by excipients. Most studies have focused on changes in chemical profiles of processed CHMs. The application of new technologies such as NMR, GC–MS and LC–MS has greatly facilitated the qualitative and quantitative analysis of herbal constituents, even at trace concentrations [41, 113,114,115]. Due to the changed chemical profiles, the finding of chemical markers that are pharmacologically relevant is essential for evaluating the processing practice. Several studies have demonstrated that “omics” studies are efficient and may at least partially represent holistic perspectives [116,117,118,119]. In a recent report, targeted glycomics and untargeted metabolomics were used to investigate the overall chemical characterization of Rehmanniae Radix . The obtained data were further processed by multivariate statistical analysis. Finally, the processing-induced chemical transformation was summarized to evoke the mechanism behind processing. In another study, metabolomics study revealed seven chemical markers of raw and processed Atractylodis Macrocephalae Rhizoma . However, despite these advances, most studies do not investigate the association of chemical and pharmacological changes. It is always valuable to assess the contribution of alteration of chemical compositions and formation of novel compounds to changed bioactivities of a CHM.
As mentioned above, decoction pieces are the only form directly applied in clinical practices. However, many studies have used the raw herb, instead of the decoction pieces, for chemical and pharmacological evaluations, which do not take into consideration of the chemical changes during processing of CHMs. This would possibly or sometimes inevitably lead to bias in understanding the traditional use of CHMs. Therefore, it is essential to use decoction pieces, especially the processed ones, for modern CHM researches.
Taken together, standardization of processing methods of CHM is a prerequisite to maintain the quality and guarantee the safety of CHM. To set up unified and scientific processing practices of CHM, further efforts should be paid to elucidate the mechanism of processing using advanced and comprehensive technologies.
Chinese herbal medicine
PR Praeparatum cum Zingibere et Alumine
PR Praeparatum cum Alumine
Traditional Chinese Medicine
Sheridan H, Kopp B, Krenn L, Guo D, Sendker J. Traditional Chinese herbal medicinal preparation: invoking the butterfly effect. Science. 2015;350:S64–6.
Wang S, Wu X, Tan M, Gong J, Tan W, Bian B, Chen M, Wang Y. Fighting fire with fire: poisonous Chinese herbal medicine for cancer therapy. J Ethnopharmacol. 2012;140:33–45.
Commission. Chinese Pharmacopoeia. 2015 edition. Beijing: China Medical Science Press; 2015.
Guo P, Brand E, Zhao Z. Chinese medicinal processing: a characteristic aspect of the ethnopharmacology of Traditional Chinese Medicine. In: Heinrich M, Jäger AK, editors. Ethnopharmacology, ch26. Hoboken: Wiley; 2015. p. 303–16.
Zhang ZH, Zhao YY, Cheng XL, Dai Z, Zhou C, Bai X, Lin RC. General toxicity of Pinellia ternata (Thunb.) Berit. in rat: a metabonomic method for profiling of serum metabolic changes. J Ethnopharmacol. 2013;149:303–10.
Yu H, Pan Y, Wu H, Ge X, Zhang Q, Zhu F, Cai B. The alum-processing mechanism attenuating toxicity of Araceae Pinellia ternata and Pinellia pedatisecta. Arch Pharm Res. 2015;38:1810–21.
Zhao Z, Liang Z, Chan K, Lu G, Lee EL, Chen H, Li L. A unique issue in the standardization of Chinese materia medica: processing. Planta Med. 2010;76:1975–86.
Sionneau P. Pao Zhi: an introduction to the use of processed Chinese medicinals. Boulder: Blue Poppy Enterprises Inc.; 1995.
Fei C, Dai H, Wu X, Li L, Lu T, Li W, Cai B, Yin W, Yin F. Quality evaluation of raw and processed Crataegi Fructus by color measurement and fingerprint analysis. J Sep Sci. 2017. https://doi.org/10.1002/jssc.201700575.
Zhan JY, Zheng KY, Zhu KY, Bi CW, Zhang WL, Du CY, Fu Q, Dong TT, Choi RC, Tsim KW, Lau DT. Chemical and biological assessment of Angelicae Sinensis Radix after processing with wine: an orthogonal array design to reveal the optimized conditions. J Agric Food Chem. 2011;59:6091–8.
Zhang Z, Wang Z, Sun S, Li J, Zhang G, Miao M. Pharmacological action of various processed Mylabris phalerata Pallas. China J Chin Meteria Med. 1990;15:214–7.
Zhou Y, Lei H, Li F, He F, Bai D, Zhou C. Discussion of processing principle of Dangshen. World Chin Med. 2009;4:161–3.
Zhang L, Ma WF, Li J, He J, Zhang P, Zheng F, Zhang BL, Gao XM, Chang YX. Influence of processing on pharmacokinetic of typical constituents in radix polygoni multiflori after oral administration by LC-ESI-MS/MS. J Ethnopharmacol. 2013;148:246–53.
Liang Z, Chen H, Yu Z, Zhao Z. Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry. Chin Med. 2010;5:29.
Feng XD, Gao GW, Huang HX. Research on the quality changes in pre-and-post-processed pieces of radix polygalae. J Chin Med Mater. 2008;31:818–20.
Yuan Z, Liu H, Wang J, Jia T, Chen J. Optimization of bran-roasted processing technology of sliced myristicae semen by orthogonal test. Chin J Inf TCM. 2016;23:74–6.
Wen JX, Zhao D, Deng J. Influence of processing methods on the chemical composition of the essential oil from Aucklandia lappa. J Chin Med Mater. 2012;35:1397–401.
Hao Y, Zifeng P, Yufeng Z, Fengrui S, Zhiqiang L, Shuying L. Analysis of norditerpenoid alkalods in processing radix aconiti lateralis preparata with radix glycyrrhizae preparata by electrospray ionization tandem mass spectrometry. Chin J Anal Chem. 2007;35:959–63.
Wu W, Liang Z, Zhao Z, Cai Z. Direct analysis of alkaloid profiling in plant tissue by using matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom. 2007;42:58–69.
Wang J, van der Heijden R, Spijksma G, Reijmers T, Wang M, Xu G, Hankemeier T, van der Greef J. Alkaloid profiling of the Chinese herbal medicine Fuzi by combination of matrix-assisted laser desorption ionization mass spectrometry with liquid chromatography-mass spectrometry. J Chromatogr A. 2009;1216:2169–78.
Mao ZC, Peng ZS. Progress on research of rapid propagation system of Pinellia ternata. China J Chin Materia Med. 2003;28:193–5.
Zhong LY, Wu H, Zhang KW, Wang QR. Study on irritation of calcium oxalate crystal in raw Pinellia ternata. China J Chin Materia Med. 2006;31:1706–10.
Wu H, Ge X, Yu H, Chen L. Comparisons of crystal form of raphides to toxicity raphides in four poisonous herbs of Araceae family. China J Chin Materia Med. 2010;35:1152–5.
Yu H, Pan Y, Wu H, Ge X, Zhang Q, Zhu F, Cai B. The alum-processing mechanism attenuating toxicity of Araceae Pinellia ternata and Pinellia pedatisecta. Arch Pharmacal Res. 2015;38:1810–21.
Tao S, Yong T, Tsui MS, Hua Y, Fu XQ, Li T, Chi LC, Hui G, Li YX, Zhu PL. Metabolomics reveals the mechanisms for the cardiotoxicity of Pinelliae Rhizoma and the toxicity-reducing effect of processing. Sci Rep. 2016;6:34692.
Liu Y, Li L, Xiao YQ, Yao JQ, Li PY, Yu DR, Ma YL. Global metabolite profiling and diagnostic ion filtering strategy by LC-QTOF MS for rapid identification of raw and processed pieces of Rheum palmatum L. Food Chem. 2016;192:531–40.
Feng S, Meijuan Y, Yan L, Yongqing X, Li L. Comparision of the actions on blood stasis of rhubarb with different prepared methods. Pharmacol Clin Chin Mater Med. 2012;6:90–3.
Wang JB, Ma YG, Zhang P, Jin C, Sun YQ, Xiao XH, Zhao YL, Zhou CP. Effect of processing on the chemical contents and hepatic and renal toxicity of rhubarb studied by canonical correlation analysis. Acta Pharmaceutica Sinica. 2009;44:885–90.
Zheng YZ, Choi RJ, Xie HQ, Cheung AW, Duan R, Guo AJ, Zhu JT, Chen VP, Bi CW, Zhu Y. Ligustilide suppresses the biological properties of Danggui Buxue Tang: a Chinese herbal decoction composed of Radix Astragali and Radix Angelica sinensis. Planta Med. 2010;76:439–43.
Du J, Bai B, Kuang X, Yu Y, Wang C, Ke Y, Xu Y, Tzang AH, Qian ZM. Ligustilide inhibits spontaneous and agonists- or K+ depolarization-induced contraction of rat uterus. J Ethnopharmacol. 2006;108:54–8.
Suzuki A, Yamamoto M, Jokura H, Fujii A, Tokimitsu I, Hase T, Saito I. Ferulic acid restores endothelium-dependent vasodilation in aortas of spontaneously hypertensive rats. Am J Hypertens. 2007;20:508–13.
Kang KS, Kim HY, Baek SH, Yoo HH, Park JH, Yokozawa T. Study on the hydroxyl radical scavenging activity changes of ginseng and ginsenoside-Rb2 by heat processing. Biol Pharm Bull. 2007;30:724–8.
Kwon SW, Sang BH, Park IH, Kim JM, Man KP, Park JH. Liquid chromatographic determination of less polar ginsenosides in processed ginseng. J Chromatogr A. 2001;921:335–9.
Sun BS, Gu LJ, Fang ZM, Wang CY, Wang Z, Lee MR, Li Z, Li JJ, Sung CK. Simultaneous quantification of 19 ginsenosides in black ginseng developed from Panax ginseng by HPLC-ELSD. J Pharm Biomed Anal. 2009;50:15–22.
Sangmyung L, Hyunju S, Chungsig C, Hung TM, Min BS, Kihwan B. Ginsenosides from heat processed ginseng. Chem Pharm Bull. 2009;57:92–4.
Kang KS, Kim HY, Yamabe N, Yokozawa T. Stereospecificity in hydroxyl radical scavenging activities of four ginsenosides produced by heat processing. Bioorg Med Chem Lett. 2006;16:5028–31.
Ha YW, Lim SS, Ha IJ, Na YC, Seo JJ, Shin H, Son SH, Kim YS. Preparative isolation of four ginsenosides from Korean red ginseng (steam-treated Panax ginseng C. A. Meyer), by high-speed counter-current chromatography coupled with evaporative light scattering detection. J Chromatogr A. 2007;1151:37–44.
Han Q, Li S, Qiao C, Song J, Cai Z, Pui-Hay-But P, Shaw P, Xu H. A simple method to identify the unprocessed Strychnos seeds used in herbal medicinal products. Planta Med. 2008;74:458–63.
Haghi G, Hatami A, Safaei A. Hydrophilic-interaction chromatography with UV detection for analysis of strychnine and brucine in the crude seeds of Strychnos nux-vomica and their processed products. Chromatographia. 2010;71:327–30.
Choi YH, Sohn YM, Kim CY, Oh KY, Kim J. Analysis of strychnine from detoxified Strychnos nux-vomica seeds using liquid chromatography-electrospray mass spectrometry. J Ethnopharmacol. 2004;93:109–12.
Wu W, Qiao C, Liang Z, Xu H, Zhao Z, Cai Z. Alkaloid profiling in crude and processed Strychnos nux-vomica seeds by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. J Pharm Biomed Anal. 2007;45:430–6.
Zhao LN, Shi YB, Zhang ZL, Zhang BS. Comparison of total cantharidin contents in blister beetle before and after processed by HPLC. Chin J Exp Tradit Med Formul. 2010;16:39–41.
Fan H. Processing principle of common toxic components of toxic Traditional Chinese Medicines. China J Chin Med. 2014;29:1335–6.
Wang K, Chen L, Ll W, Ke H, Chang C Wang. Analysis of the sesquiterpenoids in processed Atractylodis Rhizoma. Chem Pharm Bull. 2007;55:50–6.
Li W, Wen HM, Cui XB, Zhang KW. Process mechanism of Atractylodes macrocephala and conversion of sesquiterpenes. China J Chin Materia Med. 2006;31:1600–3.
Geng L, Sun H, Yuan Y, Liu Z, Cui Y, Bi K, Chen X. Discrimination of raw and vinegar-processed Genkwa Flos using metabolomics coupled with multivariate data analysis: a discrimination study with metabolomics coupled with PCA. Fitoterapia. 2013;84:286–94.
Sung M, Li P. Chemical analysis of raw, dry-roasted, and honey-roasted licorice by capillary electrophoresis. Electrophoresis. 2004;25:3434–40.
Guo YM, Yu KF, Liu YH, Zhao JZ, Wang ZC, Zhang HB. Analysis on processing mechanism of Calamine. Chin J Chin Mater Med. 2005;30:596–9.
Zhou L, Xu C, Zhang L, Ding A. Processing mechanism of calamine. China J Chin Materia Med. 2010;35:1556–9.
Yang M, Liu Z, Su Z, Zou W. Study on mechanism of precursors transforming into indigo and indirubinin blue-genera plants. China J Chin Materia Med. 2010;35:928–31.
Liu Y, Liu Z, Song F, Liu S. Optimization of preparing process condition of kansui roots by electrospray ionization mass spectrometry. J Chin Mass Spectrom Soc. 2010;31:72–8.
Bicchi C, Appendino G, Cordero C, Rubiolo P, Ortelli D, Veuthey JL. HPLC-UV and HPLC-positive-ESI-MS analysis of the diterpenoid fraction fromcaper spurge (Euphorbia lathyris) seed oil. Phytochem Anal. 2001;12:255–62.
Shen HB, Peng GP, Xie BZ. Comparison of the content of sinalbrin in Sinapis Semen before and after the process. China J Chin Materia Med. 1987;12:18–20.
Ruan G, Li G. The study on the chromatographic fingerprint of Fructus xanthii by microwave assisted extraction coupled with GC-MS. J Chromatogr B Anal Technol Biomed Life Sci. 2007;850:241–8.
Jin X, Jia X, Sun E, Wang J, Chen Y, Cai B. Research on variation regularity of five main flavonoids contents in epimedium and processed epimedium. China J Chin Materia Med. 2009;34:2738–42.
Rui N. Action of the drug herba epimedii on the testosterone of mouse plasma and its accessory sexual organ before and after processing. China J Chin Materia Med. 1989;14:18–20.
Liu F, Zhang ZQ, Lai JY, Bei HU. Determination of four kinds of alkaloids from Rhizoma Coptis and processed Rhizoma Coptis by HPLC. Chin Tradit Patent Med. 2010;32:1925–8.
Park KD, Lee SH, Kim JH, Kang TH, Moon JS, Kim SU. Synthesis of 13-(substituted benzyl) berberine and berberrubine derivatives as antifungal agents. Bioorg Med Chem Lett. 2006;16:3913–6.
Nakatani T, Konishi T, Miyahara K, Noda N. Three novel cantharidin-related compounds from the Chinese blister beetle, Mylabris phalerata Pall. Chem Pharm Bull. 2004;52:807–9.
Huh JE, Kang KS, Ahn KS, Saiki I, Kim DH, Kim SH. Mylabris phalerlata induces apoptosis by caspase activation following cytochrome c release and Bid cleavage. Life Sci. 2003;73:2249–62.
Cheng KC, Lee HM, Shum SF, Yip CP. A fatality due to the use of cantharides from Mylabris phalerata as an abortifacient. Med Sci Law. 1990;30:336–40.
Zhang Z, Wang Z, Sun S, Li J, Zhang G. Studies on the pharmacological action of various processed Mylabris phalerata pallas. China J Chin Materia Med. 1990;15:22–5.
Honkanen RE. Cantharidin, another natural toxin that inhibits the activity of serine/threonine protein phosphatases types 1 and 2A. FEBS Lett. 1993;330:283–6.
Li W, Xie L, Zheng C, Yi Z, Sun Y, Yi M, Xu Z, Xiao H. Cantharidin, a potent and selective PP2A inhibitor, induces an oxidative stress-independent growth inhibition of pancreatic cancer cells through G2/M cell-cycle arrest and apoptosis. Cancer Sci. 2010;101:1226–33.
Eisner T, Smedley SR, Young DK, Eisner M, Roach B, Meinwald J. Chemical basis of courtship in a beetle (Neopyrochroa flabellata): Cantharidin as “nuptial gift”. Proc Natl Acad Sci USA. 1996;93:6499–503.
Liu YF, Zhao LN, Zhang ZL. Determination of cantharidin in Mylabris after processing with potash by HPLC. Chin Arch Tradit Chin Med. 2010;3:487–8.
Dandan W. Study on a new method for processing of Mylabris. ShiZhen J Tradit Chin Med Res. 1996;1:40–1.
Tian JH, Lu D, Tian B, Li JY. Cantharidinate sodium injection in the treatment of bladder cancer after surgery in 23 cases. J Tradit Chin Med. 2004;45:768.
Wang X, Hou L, Xiaoping S, Tang F. Mechanisms of Semen Crotonis Pulveratum and Rhubarb intervening CD4+ CD25+/CD4+ Treg in rats with ulcerative colitis. Pharmacol Clin Chin Mater Med. 2013;2:127–9.
Kim MS, Kim HR, So HS, Lee YR, Moon HC, Ryu DG, Yang SH, Lee GS, Song JH, Kwon KB. Crotonis fructus and its constituent, croton oil, stimulate lipolysis in OP9 adipocytes. EvidenceBased Complemen Alter Med. 2014;2014:780385.
Belman S, Troll W. The inhibition of croton oil-promoted mouse skin tumorigenesis by steroid hormones. Can Res. 1972;32:450–4.
Cai B, Qin K, Hao W, Hao C, Lu T, Zhang X. Chemical mechanism during chinese medicine processing. Prog Chem. 2012;77:637–49.
Singhuber J, Ming Z, Prinz S, Kopp B. Aconitum in Traditional Chinese Medicine-a valuable drug or an unpredictable risk? J Ethnopharmacol. 2009;126:18–30.
Xie Y, Jiang ZH, Zhou H, Xu HX, Liu L. Simultaneous determination of six aconitum alkaloids in proprietary Chinese medicines by high-performance liquid chromatography. J Chromatogr A. 2005;1093:195–203.
Chan TY. Aconite poisoning following the percutaneous absorption of Aconitum alkaloids. Forensic Sci Int. 2012;223:25–7.
Shaheen F, Ahmad M, Khan MTH, Jalil S, Ejaz A, Sultankhodjaev MN, Arfan M, Choudhary MI, Atta-ur-Rahman. Alkaloids of Aconitum laeve and their anti-inflammatory, antioxidant and tyrosinase inhibition activities. Phytochemistry. 2005;66:935–40.
Liu M, Cao Y, Lv D, Zhang W, Zhu Z, Zhang H, Chai Y. Effect of processing on the alkaloids in aconitum tubers by HPLC-TOF/MS. J Pharm Anal. 2017;7:170–5.
Nyirimigabo E, Xu Y, Li Y, Wang Y, Agyemang K, Zhang Y. A review on phytochemistry, pharmacology and toxicology studies of Aconitum. J Pharm Pharmacol. 2015;67:1–19.
Chan TY. Aconitum alkaloid poisoning related to the culinary uses of aconite roots. Toxins. 2014;6:2605–11.
Chan TY. Aconitum alkaloid poisoning because of contamination of herbs by aconite roots. Phytother Res. 2015;30:3–8.
Borcsa B, Fodor L, Csupor D, Forgo P, Th MA, Hohmann J. Diterpene alkaloids from the roots of Aconitum moldavicum and assessment of Nav 1.2 sodium channel activity of aconitum alkaloids. Planta Med. 2014;80:231–6.
Borcsa B, Fodor L, Csupor D, Forgo P, Hohmann J. Assessment of the Nav1.2 sodium channel activity of Aconitum diterpene and norditerpene alkaloids. Planta Med. 2013;79:1258.
Liu Y, Tan P, Li F, Qiao Y. Study on the aconitine-type alkaloids of Radix Aconiti Lateralis and its processed products using HPLC-ESI-MSn. Drug Test Anal. 2013;5:480–4.
Qiu XH, Jie HE. Effect on the contents of ester-type alkaloids in Radix Aconiti Laterlis Preparata by different decocting time and compatibility dosage of Radix Glycyrrhizae. Lishizhen Med Mater Med Res. 2007;12:3015–7.
Wong AS, Che CM, Leung KW. Recent advances in ginseng as cancer therapeutics: a functional and mechanistic overview. Nat Prod Rep. 2015;32:256–72.
Wang CZ, Cai Y, Anderson S, Yuan CS. Ginseng metabolites on cancer chemoprevention: an angiogenesis link? Diseases. 2015;3:193–204.
Xie CL, Li JH, Wang WW, Zheng GQ, Wang LX. Neuroprotective effect of ginsenoside-Rg1 on cerebral ischemia/reperfusion injury in rats by downregulating protease-activated receptor-1 expression. Life Sci. 2015;121:145–51.
Zhang XH. Xian-Xiang Xu. Ginsenoside Ro suppresses interleukin-1β-induced apoptosis and inflammation in rat chondrocytes by inhibiting NF-κB. Chin J Nat Med. 2015;13:283–9.
Siraj FM, Sathishkumar N, Kim YJ, Kim SY, Yang DC. Ginsenoside F2 possesses anti-obesity activity via binding with PPAR and inhibiting adipocyte differentiation in the 3T3-L1 cell line. J Enzyme Inhib Med Chem. 2015;30:9–14.
Wei W, Le S, Zhe Z, Guo Y, Liu S. Profiling and multivariate statistical analysis of Panax ginseng based on ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry. J Pharm Biomed Anal. 2015;107:141–50.
Chu C, Xu S, Li X, Yan J, Liu L. Profiling the ginsenosides of three ginseng products by LC-Q-TOF/MS. J Food Sci. 2013;78:C653–9.
Zhang HM, Li SL, Zhang H, Wang Y, Zhao ZL, Chen SL, Xu HX. Holistic quality evaluation of commercial white and red ginseng using a UPLC-QTOF-MS/MS-based metabolomics approach. J Pharm Biomed Anal. 2012;62:258–73.
Lee JI, Ha YW, Choi TW, Kim HJ, Kim SM, Jang HJ, Choi JH, Choi MH, Chung BC, Sethi G. Cellular uptake of ginsenosides in Korean white ginseng and red ginseng and their apoptotic activities in human breast cancer cells. Planta Med. 2011;77:133–40.
Xie Y, Luo D, Cheng Y, Ma J, Wang Y, Liang Q, Luo G. Steaming-Induced chemical transformations and holistic quality assessment of red ginseng derived from panax ginseng by means of HPLC-ESI-MS/MSn-based multicomponent quantification fingerprint. J Agric Food Chem. 2012;60:8213–24.
Xiao SY, Luo GA. Chemical reactions of ginsenosides in red ginseng processing by HPLC/MS/MS. Chin Tradit Herbal Drugs. 2005;1:40–3.
Lee ES, Choi JS, Kim MS, You HJ, Ji GE, Kang YH. Ginsenoside metabolite compound K differentially antagonizing tumor necrosis factor-α-induced monocyte-endothelial trafficking. Chem Biol Interact. 2011;194:13–22.
Wang CZ, Aung HH, Zhang B, Sun S, Li XL, He H, Xie JT, He TC, Du W, Yuan CS. Chemopreventive effects of heat-processed Panax quinquefolius root on human breast cancer cells. Anticancer Res. 2008;28:2545–51.
Qian XC, Zhang L, Tao Y, Huang P, Li JS, Chai C, Li W, Di LQ, Cai BC. Simultaneous determination of ten alkaloids of crude and wine-processed Rhizoma Coptidis aqueous extracts in rat plasma by UHPLC-ESI-MS/MS and its application to a comparative pharmacokinetic study. J Pharm Biomed Anal. 2015;105:64–73.
Tan HL, Chan KG, Priyia P, Acharaporn D, Surasak S, Tahir MK, Learn-Han L, Bey-Hing G. Rhizoma Coptidis: a potential cardiovascular protective agent. Front Pharmacol. 2016;7:362.
Hyunah J, Min BS, Yokozawa T, Jehyun L, Yeongshik K, Jaesue C. Anti-Alzheimer and antioxidant activities of coptidis rhizoma alkaloids. Biol Pharm Bull. 2009;32:1433–8.
Lee JY, Park NH, Lee W, Kim EH, Jin YH, Seo EK, Hong J. Comprehensive chemical profiling of Pinellia species tuber and processed Pinellia tuber by gas chromatography-mass spectrometry and liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry. J Chromatogr A. 2016;1471:164–77.
Yu HL, Mao SH, Zhao TF, Wu H, Pan YZ, Shu CY. Antagonistic effect of gingerols against TNF-α release, ROS overproduction and RIP3 expression increase induced by lectin from Pinellia ternata. China J Chin Materia Med. 2015;40:3630–5.
Pan X, Bligh SW, Smith E. Quinolone alkaloids from Fructus Euodiae show activity against methicillin-resistant Staphylococcus aureus. Phytother Res. 2014;28:305–7.
Yin YY, Liu SS, Han LW, Qiu-Xia HE, Zhang QW, Liu KC, Yan LH, Wang ZM. Chemical components of alkaloids from euodiae fructus and their anti-angiogenic activities. Chin J Exp Tradit Med Formul. 2016;22:45–53.
Kano Y, Qine Z, Komatsu K. On the evaluation of the preparation of Chinese medicinal prescriptions. VI. The changes of the alkaloid contents by processing of Evodia fruit, Yakugaku Zasshi. J Pharm Soc Japan. 1991;111:32–5.
Guo P, Brand E, Zhao Z. Chinese medicinal processing: a characteristic aspect of the ethnopharmacology of Traditional Chinese Medicine. Hoboken, New Jersey, United States: Wiley; 2000. p. 132–40.
Zhang L, Shu X, Tang Y, Ding A, Duan J. Study on preparation processing technique of Radix Kansui stir baked with vinegar. China J Chin Mater Med. 2009;34:681–4.
Mubai S, Zhu J. Processing technology and quality of red ginseng. J Changchun Univ Chin Med. 2014;30:611–3.
Zhangchi N, Zhiqian S, Chun W, Yuanyan L, Honglian Z, Jiahe G, Xinling M, Zhenli L. Effects of processed temperature and time on color and contents of six types of Boswellic acids in Frankinense. Mod Tradit Chin Med Mater MedicaWorld Sci Technol. 2017;19:508–15.
Chen K, Yuan J. The effects of processing the Coptis chinensis using different types of wine on the main components of alkaloids. Adv Anal Chem. 2016;6:14–9.
Zhu X, Wang C, Wang X, Cai M, Deng S. Effects of different processing methods on the determination of trigonelline in Fructus Cannabis decoction pieces. J Pharm Res. 2016;35:19–21.
Liu LH. Optimization of microwave extraction process of total alkaloid from Aconitum flavum. Med Plant. 2010;1:93–5.
Wu X, Zhu L, Ma J, Ye Y, Lin G. Adduct ion-targeted qualitative and quantitative analysis of polyoxypregnanes by ultra-high pressure liquid chromatography coupled with triple quadrupole mass spectrometry. J Pharm Biomed Anal. 2017;145:127–36.
Guo S, Duan JA, Qian D, Wang H, Tang Y, Qian Y, Wu D, Su S, Shang E. Hydrophilic interaction ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry for determination of nucleotides, nucleosides and nucleobases in Ziziphus plants. J Chromatogr A. 2013;1301:147–55.
Hankemeier T. Traditional processing strongly affects metabolite composition by hydrolysis in Rehmannia glutinosa roots. Chem Pharm Bull. 2011;59:546–52.
Li Z, Xu JD, Zhou SS, Qian M, Ming K, Hong S, Li XY, Duan SM, Xu J, Li SL. Integrating targeted glycomics and untargeted metabolomics to investigate the processing chemistry of herbal medicines, a case study on Rehmanniae Radix. J Chromatogr A. 2016;1472:74–87.
Zhang CE, Niu M, Li Q, Zhao YL, Ma ZJ, Xiong Y, Dong XP, Li RY, Feng WW, Dong Q. Urine metabolomics study on the liver injury in rats induced by raw and processed Polygonum multiflorum integrated with pattern recognition and pathways analysis. J Ethnopharmacol. 2016;194:299–306.
Shan GS, Zhang LX, Zhao QM, Xiao HB, Zhuo RJ, Xu G, Jiang H, You XM, Jia TZ. Metabolomic study of raw and processed Atractylodes macrocephala Koidz by LC–MS. J Pharm Biomed Anal. 2014;98:74–84.
Sun H, Ni B, Zhang A, Wang M, Dong H, Wang X. Metabolomics study on Fuzi and its processed products using ultra-performance liquid-chromatography/electrospray-ionization synapt high-definition mass spectrometry coupled with pattern recognition analysis. Analyst. 2012;137:170–85.
SW and CH designed the study. JL and JC conducted the literature search. XW and SW drafted the manuscript and prepared tables and figures. YJ, ML and BB contributed to revisions of the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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Wu, X., Wang, S., Lu, J. et al. Seeing the unseen of Chinese herbal medicine processing (Paozhi): advances in new perspectives. Chin Med 13, 4 (2018). https://doi.org/10.1186/s13020-018-0163-3
- Chinese herbal medicines
- Decoction pieces