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  • Review
  • Open Access

Indigenous medicinal plants of Pakistan used to treat skin diseases: a review

  • 1Email author,
  • 1,
  • 1,
  • 1,
  • 2,
  • 3 and
  • 4
Chinese Medicine201813:52

  • Received: 13 May 2018
  • Accepted: 11 October 2018
  • Published:


Ethno-pharmacological relevance

Plants are providing reliable therapy since time immemorial. Pakistan has a great diversity in medicinal flora and people use these ethno-medicines to deal with many skin problems. This review explores the fundamental knowledge on various dermatological properties of medicinal plants of Pakistan and is aimed to provide a baseline for the discovery of new plants having activities against skin issues.

Material and method

A total of 244 published articles were studied using different research engines like PubMed, Google, Google-scholar and science direct.


Review of literature revealed ethno-pharmacological use of 545 plant species, belonging to 118 families and 355 genera, to combat various skin ailments. Out of these, ten most commonly used plant species belonging to ten different families are documented in this review. It was also found out that ehno-medicines are prepared using various parts of the plants including leaves (28.32%), whole plant and roots 13.17% and 10.97% respectively, in the form of powder (23.5%) and paste (22.75%). A total of 13 endangered plant species and ten commercially important plants were recorded.


Medicinal plants of Pakistan have therapeutic effects against several skin problems; however most of medicinal plants are still not evaluated scientifically to support their ethno-pharmacological claim on skin. Dermatological pathogens are recommended to study. Further, the conservational programs should be established for endangered species.


  • Medicinal flora
  • Skin problems
  • Ethno-medicine
  • Dermatological pathogens


History of natural products is as old as human civilizations and so is the Indigenous knowledge, which is handed down to the people from their ancestors through verbal communication; people have been living in close association with plants since time immemorial [1]. The purpose of standardizing traditional remedies is, obviously, to ensure therapeutic efficacy of medicinal plants; whereas the value of ethno-medicinal information in modern pharmacology lies in the development of new drugs. Some modern drugs have been deducted from folklore and traditional medicines [2]. The origin of the word “Ethnobotany” is accredited to US ethnobotanist “John Harshberger” who described and explained the relationship between people and plants they use in a culture in 1895 [1, 3]. In Sub-continent, Rig Veda (4500–1600 BC) compiled first record of ethno-medicine [4].

In ancient times, natural products were successfully used to treat different ailments owing to their enhanced acceptability in human society, better compatibility with the body and their natural power to treat ailment via synergistic effects and neutralizing combinations to lessen adverse effects [5]. Medicinal plants are better as compared to synthetic drugs because of minimal adverse reactions [6, 7]. Medicinal plants (trees, shrubs, grasses or vines) can be used in different forms [8] like extracts, in fresh or powdered form, seeds, fruits, vegetable mixtures, etc. [9].

According to an estimate, earth carries 265,000 species of plants but only half of these are yet investigated for their medicinal values and chemical composition. In developing countries, around 80% of the population depends upon medicinal plants for combating different diseases but this was estimated about a decade ago [10, 11] while in developed countries, 60% of the population uses these plants [6], 40–50% of the population in Germany, 42% in the USA, 48% in Australia and 49% in France depends upon plants for different health issues [12]. Importance of these medicinal plants can be judged by the fact that at least 25% of the drugs enlisted in modern Pharmacopoeia are of plant origin [5]. Also about 25% of the medical prescriptions are based on the substances or analogs of the substances of plant origin [13].

Pakistan occupies 80,943 km2 area and lies between 60°55′ to 75°30′ E longitude and 23°45′ to 36°50′ N latitude [14], and has altitude ranges from 0 to 8611 m with a mixed climate zones; has a large biodiversity of the medicinal plants. Pakistan is blessed with 6000 species of higher plants, of which 600–700 are used medicinally [15], out of these 6000 species, half (3000 species) are reported from Northern areas out of which 124 species have medicinal importance [16, 17], 4940 flowering plants are native to Pakistan (if cultivated flowering species are included figure turns 5738) [14]. Unfortunately only 10% of the total plant species in Pakistan have reported medicinal values [18].

In Pakistan, a large population uses folk medicines and it has become a definite part of its cultural heritage [19]. In early 1950s, most of the health concerned issues were treated using traditional indigenous experiences by more than 84% of the population of Pakistan but now this practice is limited to only remote areas of the country [6, 20, 21]. In 1958, Hocking also reported same percent (84%) of Pakistan population depending upon traditional medicinal plants for treating different ailments. In 1983, 63% of the population in Pakistan, especially in villages, was reported to use herbal medicines prescribed by the traditional prescribers [22]. Knowledge on the use of medicinal plants is enormous but if this traditional knowledge is not rapidly researched and recorded, indications are that it will be lost with succeeding generations [23].

Skin, the most diverse organ of the human body, is very important for aesthetic reasons and health issues. Skin diseases not only give unfavorable looks but also pushes the patient into psychic conditions [24]. It has been estimated that skin diseases account for 34% of all occupational disease [25]. Despite of all the developments in the medical science, it is still complicated to manage skin diseases specifically in developing countries, due to the fact that health care workers lack training in skin care and skin diseases, which have been of major concern, recently, due to their association with the Human Immunodeficiency Virus and Acquired Immunity Deficiency Syndrome (HIV/AIDS) [26]. Since human civilization, plants are used to treat major skin issues like wounds, cuts and burns [27]. In a study, it was documented that 80% of the Indian population uses ethno-medicines to deal with skin problems and 50% of the medicinal plants used against skin problems are restricted to forest [28].

This review is an attempt to summarize utmost possible information on ethno-medicines and pharmacology of the plants used in Pakistan to cure skin diseases. This study was aimed to investigate the ethno-medicinal uses of the plants of Pakistan to treat various skin conditions. We were interested in gathering the answers of the following questions in particular: Which plant species are most commonly used traditionally for skin problems? Which skin conditions are most commonly treated using ethno-medicine? Which parts of medicinally important flora of Pakistan are used against skin diseases? What are the recipes for preparing and applying the ethno-medicines? Above all, the review will identify gaps in the current knowledge that will provide a baseline for further research activities. Also, the review is aimed to highlight the area of Pakistan still need to be investigated.

Data collection and analysis

Published papers were retrieved from the online bibliographical database latest till June 2015, search engines included: PubMed, Google, Google.scholar, IUNCredlist, druginfosys and sciencedirect. Inside the database, we used the keywords like traditional plants for skin, ethnobotany of Pakistan, ethno-medicine, traditional uses of plants, indigenous plant knowledge, plants used in ethno-pharmacology. While reviewing the literature, focus was on the plants with the potential traditional usage against various diseases of skin. In total, 244 articles on Indigenous plants of Pakistan published in English language were reviewed for this study for the period March 2015 to December 2017. Articles selected for this review contain plants that were (i) native to Pakistan with some wide distribution (ii) traditionally used in Pakistan for treating skin diseases; only those plants were selected that have ethno-pharmacological evidence for skin treatment. A master document was formulated enlisting indigenous medicinal plants of Pakistan used by the local inhabitants for the treatment of several skin ailments. Master list constituted vernacular name of the medicinal plants along with the family name, part used, mode of preparation, life form and skin conditions against which the plant is being used. All the data were summarized into 6 tables and 9 figures.

As this review is written after consulting a large number of articles, only references are provided in it due to vey diversified data.

The data collected were grouped into 26 categories on the basis of skin conditions; 28 categories based on families plant belong to; 7 categories based on mode of preparation of medicine, 30 categories based on the part used, 9 categories based on habit of plants and 6 categories based on the region the plant grows.

Plants reported in more than one region of Pakistan were enlisted only once for making the final list of medicinal plants. Conservation status was evaluated following IUCN red list categories version 3.1 and the herbal products registered in Pakistan and economical importance of the plants was estimated by consulting and scientific literature respectively.

Taxonomic problems

Several taxonomic problems were observed in the development of this manuscript. Entirely, we documented 10 plant species most frequently used by the people of Pakistan against skin diseases, there were some spelling mistakes in family names, botanical names, publication and authors, which were also verified according to “”, “” and “”.

Results and discussion

The present review revealed ethno-medicinal use of 545 plant species belonging to 118 families and 355 genera to cure various skin diseases, 10 plant species belonging to different genera and families which were most commonly used in different regions of Pakistan against several skin ailments are documented in this review (Table 1).
Table 1

List of plants used traditionally against skin conditions

Sr. no

Botanical name

Common name



Part used


Folk claim


Pharmacological validation



Calotropis procera (Aiton) R. Br.

Dasi ak, Milk weed, Akk, Spalmaka, wallow-wort, Sodom apple, Dead Sea apple



St, bk, lf, sd, rt, fr, wp

(1) Decoction of stem and leaves is taken

(2) Latex is mixed with castor oil and applied

(3) Paste with raw sugar is applied over the dog bitten wounds

(4) Flowers are put in oil & applied on wounds

(5) Crushed leaves

(6) The leaves are warmed and tied over the wounds and used as poultice

(7) Roots are powdered, mixed with “desi ghee” and pasted on points of leprosy

Leprosy, wound healing, abscess, ringworm, dog bitten wounds, eczema, pustules and pimples, skin eruptions, syphilis, boils, ulcer, burn, dermatitis, scabies, infection and other skin diseases

[3, 40, 5864]

Anti-hyperbilirubinemic and wound healing activity of aqueous extract of Calotropis procera leaves in Wistar rats [89]

Calotropis procera extract induces apoptosis and cell cycle arrest at G2/M phase in human skin melanoma (SK-MEL-2) cells [90]

Wound-healing and potential anti-keloidal properties of the latex of Calotropis procera (Aiton) Asclepiadaceae in rabbits [91]

Wound healing in diabetes mellitus: traditional treatment modalities [92]

Healing potential of Calotropis procera on dermal wounds in Guinea pigs [93]

Procerursenyl acetate, proceranol, N-dotriacont-6-ene, glyceryl mono-oleolyl-2-phosphate, methyl myrisate, methyl behenate and glyceryl-1,2-dicapriate-3-phosphate [115]


Berberis lyceum Royle

Sumblu, komal, Kowdach, Berberry, Churku, Ishkeen, Ishkin (Urdu), Ishkein (Shina), Sumbal



Rt, st, br, lf, fr

(1) The paste of root and bark is externally applied on wounds

(2) Crushed bark is soaked in water and the extract is taken in morning to treat scabies, boils and pimples

(3) Decoction

(4) Dried root mixed with egg and fried in cow’s ghee is used

(5) Dried powdered root is spread over external wounds

(6) For external wounds peel off an epidermis of root and boil the inner cortex

(7) Bark powder paste mixed with mustard oil is used

Gonorrhea, wound healing, ulcers, Scabies, Boils, Pimples

[18, 6567]

Wound healing activity of root extracts of Berberis lyceum royle in rats [94]

Berberine, ß-sitosterol, 4,4-dimethylhexadeca-3-ol, butyl-3-hydroxypropyl phthalate, 3-(4′-(6-methyl butyl) phenyl)p ropan-1-ol, 4-methyl-7-hydroxycoumarin [116]


Dodonaea vescosa (L.) Jacq.

Ghwara-sky, Sanatha, Anartirk/Hanartirk



Wp, Lf, sd, Bk, wd, rt, fl

(1) Paste of dried powdered leaves and water is applied

(2) Fresh leaves are crushed to the extent to become sticky and then tied on the effected part of the body for wounds healing

(3) Poultice

(4) Leaves are grinded, mixed in water and bath is taken with this water

(5) Decoction

(6) Dry leaves are tied on wounds and used for softening of wound

Swelling, Germicidal, pimples, Burn and wound healing, cracked skin, rashes, itching and pustules, allergy

[40, 58, 60, 6770]

Antifungal activity of Dodonaea viscosa Jacq extract on pathogenic fungi isolated from superficial skin infection [95]

Toxicity studies on dermal application of plant extract of Dodonaea viscosa used in Ethiopian traditional medicine [96]

Antifungal activity of the plant Dodonaea viscosa var. Angustifolia on Candida albicans from HIV-infected patients [97]

Sakuranetin, leucocynindns, quercetin, kaempferol, isorhamnetin, 5,7,4′-trihydroxy-3′-(3hydroxymethylbutanol) 3,6-dimethoxyflavone, 5,7-dihydroxyflavanone, 5,4′-dihydroxy-3,6,7-trimethoxy flavone, 3′-(γ,γ-dimethyalllyl)-5,7-dihydroxy-3,6,4′-trimethoxyflavone, 3,5,7,4′-Tetrahydroxyl-3,4′-dimethoxyflavone, 5,4′-dihydroxy-6,7-dimethoxyflavone, 5,7-dihydroxy-6, 4′-dimethoxyflavone, 3,5-dihydroxy-7,4′-dimethoxyflavone, 5-hydroxy-3,7,4′-trimethoxyflavone, trimethoxy flavone, 5 hydroxy-7,4′-dimethoxyflavone, 6 Hydroxy-3,6,7 trimethoxyflavone, 5,7,4′ Trihydroxy-3-methoxyflavone [117]


Achyranthes aspera L.

Apang, Puth Kanda, Prickly flower, Jishkay



lf, st, sd, wp

(1) Ash of leaves and stem

(2) Juice

(3) Decoction

(4) Paste of leaves

leprosy, Itching, Skin eruptions and irritation, abscess and boils, ulcer and other Skin diseases

[3, 36, 64, 68, 7173]

Cancer chemopreventive activity of Achyranthes aspera leaves on Epstein-Barr virus activation and two-stage mouse skin carcinogenesis [98]

Antibacterial activities of selected medicinal plants in traditional treatment of human wounds in Ethiopia [99]

In vivo wound-healing efficacy and antioxidant activity of Achyranthes aspera in experimental burns [100]

Evaluation of in vivo wound healing activity of methanol extract of Achyranthes aspera L. [101]

Anti-herpes virus activities of Achyranthes aspera: an indian ethnomedicine, and its triterpene acid [102]

Pharmacological evaluation and chemical standardization of an ayurvedic formulation for wound healing activity [103]

α-l-Rhamnopyranosyl-(1 → 4)-(β-d-glucopyranosyluronic acid)-(1 → 3)-oleanolic acid, α-l-rhamnopyranosyl-(1 → 4)-(β-d-glucopyranosyluronic acid)-(1 → 3)-oleanolic acid-28-O-β-d-glucopyranoside and α-l-rhamnopyranosyl-(1 → 4)-(β-d-glucopyranosyluronic acid)-(1 → 3)-oleanolic acid-28-O-β-d-glucopyranosyl-(1 → 4)-β-d-glucopyranoside, n-hexacos-14-enoic acid, strigmasta-5, 22-dien-3-β-ol, trans-13-docasenoic acid, n-hexacosanyl, n-decaniate, n-hexacos-17-enoic acid and n-hexacos-11-enoic acid, 36, 37-dihydroxyhenpentacontan-4-one and Triacontanol, β-d-glucopyranosyl3β-[O-α-l-rhamnopyranosyl-(1 → 3)-O-β-d-glucopyranuronosyloxy]machaerinate, β-d-glucopyranosyl3β-[O-β-d-galactopyranosyl-(1 → 2)-O-α-d-glucopyranuronosyloxy]machaerinate, β-d-glucopyranosyl-3β[O-α-l-rhamnopyranosyl-[1 → 3)-O-β-d-glucopyranuronosyloxy]oleanolate, β-d-glucopyranosyl3-β-[O-β-d-galactopyranosyl (1 → 2)-O-β-d-glucopyranuronosyloxy] oleanolate, β-d-glucopyranosyl 3β-[O-β-d-glucopyranuronosyloxy]

Oleanolate [118]


Nerium oleander L.

Kaner, Gndeer, Ganaera, Gandeera



Rt, Lf, Bk, Br

(1) Oil extracted from the root and bark

(2) Paste

(3) Leaves with honey used as a poultice

(4) Decoction

Scabies, ulcers, leprosy and scaly skin, Gangrine, maggots infesting wounds


Antioxidant, anti-inflammatory, anti-apoptotic, and skin regenerative properties of an Aloe vera-based extract of Nerium oleander leaves [104]

Nériine, digitoxigénine, Amorphane, 1.8-cineole, α-pinene, calarene, Limonene, βPhellandrene, Terpinene-4-ol, sabinene, Isoledene, 3-Carene, Humulene, β-Pinene and Cymen-8-ol [119]


Riccinus communis L.

Arand, Raned, Hurnoli



Wp, Sd, lf, bk, rt

(1) Grinded leaves

(2) Oil obtained from the seeds

(3) Paste of leaves is slightly warmed over fire and applied

Freckles, scabies, wounds and sores healing, boils, acne, leprosy, ringworm, swelling, warts removal and other skin diseases


Some Nigerian plants of de rmatologic importance [105]

Antimicrobial activity of Palestinian medicinal plants against acne-inducing bacteria [106]

In vitro assessment of cytotoxicity, antioxidant, and anti-inflammatory activities of Ricinus communis (Euphorbiaceae) Leaf Extracts [107]

Natural pharmacopoeia used in traditional Toba medicine for the treatment of parasitosis and skin disorders (Central Chaco, Argentina) [108]

Effect of Solanum nigrum and ricinus communis extracts on histamine and carrageenan-induced inflammation in the chicken skin [109]

Ricinine, N-demethylricinine, glycosides kaempferol-3-O kaempferol-3-O-β-d-glucopyranoside, quercetin

Xylopyranoside, quercetin-3-O-β-d-glucopyranoside, kaempferol

O-β-rutinoside and quercetin-3-O-β rutinoside, 1, 8-cineole, camphor and α-pinine (β-caryophyllene, ricinoleic, isoricinoleic, stearic and dihydroxystearic acids, ricinine, ester form of palmitic, stearic, arachidic, hexadecenoic, oleic, linoleic, linolenic, ricinoleic (89.4%) and dihydroxy stearic acids, ergost—en-3-ol, stigmasterol, Y-sitosterol, fucosterol; and probucol [120]


Carthamus tinctorious L.

Pome, Pong, Tukhmigartum, Safflower



Fl, sd, lf

(1) Powder

(2) Decoction

(3) Paste

(4) Juice

Chicken pox, measles and eruptive skin problems

[17, 79]

Inhibitory effects of active compounds isolated from safflower (Carthamus tinctorius L.) Seeds for melanogenesis [110]

Kinetic study on the tyrosinase and melanin formation inhibitory activities of carthamus yellow isolated from Carthamus tinctorius L. [111]

Inhibitory effect of hydroxysafflor yellow a on mouse skin photoaging induced by ultraviolet irradiation [112]

Enzyl-O-β-d-glucopyranoside, syringarenol, lirioresinol-A, 5-hydroxymethyl-2-furaldehyde, β-sitosterol, and stigmasterol [121]


Solanum nigrum L.

Mako, Black Nightshade



Wp, Ber, lf, Ft, Lf, st

(1) The juice of the ripen berries is applied on the skin

(2) Poultice

(3) Crushed leaves

(4) Fruits are crushed and applied externally

(5) Decoction

Cosmetics, dried skin, pimples, freckles, as sun block, corrosive ulcer and suppurating Syphilitic ulcer, Pustules, ring worms, wounds healing, eczema, leukoderma

[18, 78, 8083]

Effect of Solanum nigrum and Ricinus communis extracts on histamine and carrageenan-induced inflammation in the chicken skin [109]

(+)-pinoresinol (I), (+)-syringaresinol (II), (+)-medioresinol (III), scopoletin (IV), tetracosanoic acid (V) and beta-sitosterol [122]


Albizia lebbeck (L.) Benth

Siris, Shareen



Lf, sd, bk, fl, st, wd, tr

(1) Dried leaves are smoked

(2) Paste of flower

(3) Extract

Wound healing, leucoderma, itching, Inflammations, boils, eruption

[78, 84]

Wound-healing potential of the root extract of Albizzia lebbeck [113]

Budmunchiamines L1–L3, Quercetin, kaempferol, 3-O-α-rhamnopyranosyl (1 → 6)-β-glucopyranosyl(1 → 6)-β-galactopyranosides and Albiziasaponins A, B and C [123]


Plantago lanceolata L.

Bar-e-Thang, Boieko-ligini, Isphaghol, Ghwa jabai



Lf, ft, sd, rt

(1) Fresh leaves are mashed and put on wounds

Wound and burn healing, Skin sores, inflamed surfaces, bruises

[3, 4, 66, 8588]

Plantago lanceolata L. Water extract induces transition of fibroblasts into myofibroblasts and increases tensile strength of healing skin wounds [114]

catalpol, aucubin, and acteoside [124]

H, herb; S, stem; T, tree; Bk, bark; Lf, leaf; sd, seed; rt, root; Wp, whole plant; ber, berries; fl, flower; br, branch

Distribution of plants in different regions of Pakistan

Pakistan holds rich diversity of medicinal plants used against various ailments. The present review reported 545 plants from different regions of Pakistan being ethno-medicinally used for treating various skin problems. Majority of 278 plants were reported from Punjab belonging to 78 families followed by 204 plants from Gilgit from 73 families, 201 from Kashmir belonging to 74 families, 187 from KPK from 73 families, 47 from Sindh from 27 families and 25 from Balochistan belonging to 14 families. Many regions are still un-investigated; a list of investigated as well as un-investigated areas of Pakistan is given in Table 2. Many of the plants and hence families were used in more than one region; those plants and their families were counted just one time when enlisting for overall plants of Pakistan. The distribution of plants and their families in different regions of Pakistan, according to their use in skin conditions is shown in Fig. 1.
Table 2

Investigated and under-investigated districts for ethno-botanical studies


Investigated districts for ethno-botanical studies

Under-investigated districts for ethno-botanical studies


Attock, Bahawalnaga, Bahawalpur, Bhakkar, Chakwal, Dera Ghazi Khan, Faisalabad, Gujranwala, Gujrat, Jhang, Jhelum, Kasur, Khushab, Mianwali, Multan, Muzaffargarh, Narowal, Nankana Sahib, Pakpattan, Rajanpur, Rawalpindi, Sahiwal, Sargodha, Sialkot, Toba Tek Singh, Vehari

Chiniot, Hfizabad, Khanewal, Okara, Rahim Yar Khan, Sheikhupura

Khyber Pakhtunkhwa

Abbottabad, Bannu, Battagram, Buner, Chitral, Dera Ismail Khan, Haripur, Karak, Kohat, Upper Kohistan, Lakki Marwat, Lower Dir, Malakand, Mansehra, Peshawar, Swat, Upper Dir, Lower Kohstan

Charsadda, Hangu, Mardan, Nowshera, Shangla, Swabi, Tank, Tor Ghar


Ghotki, Jamshoro, Karachi, Kairpur, Sanghar, Sukkur, Tharparkar, Thatta, Karachi West

Badin, Dadu, Hyderabad, Jacobabad, Kashmore, Larkana, Matiari, Mirpurkhas, Naushahro Firoze, Shaheed Benazirabad. Kambar, Shahadkot, Shikarpur, Tando Allahyar, Tando Muhammad Khan, Umerkot, Sujawal, Karachi Central, Karachi East, Karachi South, Korangi, Malir


Ghanche, Skardu, Astore, Diamer, Ghizer, Gilgit, Hunzanagar


Gilgit Baltistan

Muzaffarabad, Hattian, Neelum, Mirpur, Bhimber, Kotli, Poonch, Bagh, Haveli, Sudhnati

Fig. 1
Fig. 1

Distribution of medicinal plants in different regions of Pakistan

Medicinally important families and genus against skin ailments

A total number of 118 families were studied and out of them 10 families were documented in this review. Among these families, Asteraceae (8.99%) is the most commonly used family followed by Fabaceae (4.58%), Poaceae (3.73%), Laminaceae and Ranunculaceae (3.39% each), Rosaceae (3.22%), Euphorbiaceae and Solanaceae (3.05%), Polygonaceae (2.88%), Boraginaeae (2.71%), Papilionaceae and Amaranthaceae (2.37% each), Apocynaceae (2.20%), Cucurbitaceae and Malvaceae (1.86.5% each), Chenopodiaceae, Salicaceae and Liliaceae (1.69% each), Apiaceae and Brassicaceae (1.52% each), Moraceae, Scrophulariaceae and Zygophyllaceae (1.35% each) Capparidaceae, Convolvulaceae and Rutaceae (1.18% each), Cyperaceae, Rhamnaceae, Saxifragaceae and Tamaricaceae (1.01% each) and some other 87 families (30.39%).

The results, in terms of percentage, of commonly used families are represented in Fig. 2.
Fig. 2
Fig. 2

Families of plants active against skin ailment

Asteraceae holds the top position among the families used in ethno-medicines which indicates the presence of effective bioactive ingredients in the members of this family [29]. Lamiaceae and Asteraceae are the most frequently used families in ethno-medicine [30]. It was reported that Asteraceae family is the most diverse family found in all the habitat and regions except in Antarctica and it was not a new finding about Asteraceae family holding highest position among the traditionally important families, it was further concluded that this prevalence is due to high quantities of active secondary metabolites present in this family and also because this family includes a large number of species [31].

Euphorbia is the genera having highest number of species (11 ssp.) used to fight skin problems followed by Artemisia with 9 ssp., Ficus and Salix (8 ssp. each), Solanum (7 ssp.), Impatiens, Polygonum, Rumax, Saussurea, Ziziphus (6 ssp. each), Clematis, Datura, Tamarix, Vernonia (5 ssp. each), 15 genera with 4 ssp., 23 genera with 3 ssp., 72 genera with 2 ssp. and 224 genera with 1 ssp. used to combat different skin ailments.

Medicinally important genera along with the number of species used effectively against skin ailments in terms of percentage are graphically represented in Fig. 3.
Fig. 3
Fig. 3

Common genera used for skin diseases

Euphorbia taking the top place in the genera used most frequently in ethno-medicine due to the reason that it is the largest genus of family Euphorbiaceae with 1600 reported species [32].

Common skin diseases in Pakistan

In this review, it was found out that most of the plant species were used to treat more than 1 disease/condition of skin. Total 26 skin conditions were reviewed which were treated using ethno-medicine. Most common of them was wound healing (17.064%) followed by boil healing (8.72%), postules or pimples (5.83%), eczema (5.01%), ulcer (4.579%), burn (4.51%), Ringworm (3.889%) and scabies (3.889%).

These most commonly treated skin conditions mentioned above are represented graphically in Fig. 4 and total of all the skin conditions treated traditionally are represented in Fig. 5.
Fig. 4
Fig. 4

Common skin diseases treated traditionally

Fig. 5
Fig. 5

All the skin diseases treated traditionally in Pakistan

Burn, which accounts 4.51% of the skin conditions treated traditionally in Pakistan is a serious issue globally, De Wet et al. reported increased mortality rate due to burn which has contributed to a serious health concerned issue globally. In South Africa, burn is one of the major reasons of deaths among 5–29 years individuals. Ethno-medicines have great potential to cure different kind of skin diseases [25, 137].

Some plants cure more than one skin disease, Curcuma longa and Melia azaderach are the natural resources for curing multiple skin diseases [33, 138].

Tabassum and Hamdani concluded that skin conditions can be of thousand types but are categorized into nine categories based on common diseases; rashes, viral infections, bacterial infections, fungal infections, parasitic infections, pigmentation disorder, tumor and cancer, trauma and other conditions (Wrinkles, spider veins and varicose veins) [28]. This review revealed that all these skin conditions are treated traditionally using etho-medicines.

Mabona and Vuuren reported wound healing as a most frequently treated skin condition using ethno-medicine, followed by sores or ulcers. Among the medicinal plants used to combat wounds, Erythrina genus is used most frequently in South Africa with 120 species having their space in the traditional usage against skin conditions particularly in disinfection of wounds. Traditional plants are also used to combat associated condition of wounds including inflammation, urticaria, skin allergies, acne, eczema and psoriasis. Among these associated conditions, eczema is most commonly diagnosed in South Africa and thus most frequently treated with ethno-medicines [34].


Growing habits of 42.23% plants were not available in the previous articles. Common life forms used by traditional healers for the preparation of ethno-medicines include Trees 9.68%, Shrub 11.52%, Herb 34.72%, Creepers and Climbers 0.5% each, Grass 0.5% (only 3 plants were a grass), Sedges 0.1% (only 1 plant was from sedges) as shown in Figs. 6 and 7.
Fig. 6
Fig. 6

Habit of plants in different regions of Pakistan

Fig. 7
Fig. 7

Habit of plants in Pakistan

Trees and shrubs are used to prepare majority of herbal recipes as they are accessible round the year, followed by utilization of herbs which might be related to their easy collection methods, higher abundance and efficacy in curing ailments as compared to other life forms [30]. Khan et al. reported shrubs and trees as most commonly used life forms in ethno-medicines due to the their availability around the year with the minimum seasonal variation [35]. Ahmad et al. stated that herb is used in ethno-medicines due to their abundance and easy availabilty in most of the aresa of the world [36].

Part used

Ethno-medicines mostly consisted of plant parts such as leaves, roots, fruits, seeds, flowers, stem, bark and some other parts of the plants listed below. Traditional knowledge on the effectiveness of different plant parts could have been established through trial and error basis along with observations such as taste, smell and texture. This study revealed that leaves (28.32%) were the most common part of indigenous plants used in different preparations of ethno-medicines for the treatment of skin diseases followed by whole plant (13.17%), roots (10.97%), fruits (9.89%), flowers (6.79%), stem (5.78%), bark (5.60%), Shoos and latex (1.37% each), aerial parts and wood (1.07% each), branches (0.89%), Berries (0.77%), rhizomes (0.71%), gum (0.65%), resins and bulb (0.41% each), pod (0.29%), pulp and tubers (0.23% each), milky acrid, nut, inflorescent, twigs (0.17% each), sap, fronts, trunk (0.11% each), husk and gall (0.05% each) (Fig. 8).
Fig. 8
Fig. 8

Part used by traditional healers to prevent different skin ailments

Leaves were the most commonly used plant part in ethno-medicine, this is due to the ease of processing them into a digestible paste and have less conservational issues than the collection of roots, bark, stem or the whole plant and also because leaves does not affect the life cycle of the plants [29], further the life form of the plant is not effected by the collection of leaves [37] and also due to the reason that leaves contain photosynthate which might have some medicinal value [38]. Ayyanar and Ignacimuthu reported leaves as most frequently used plant part (accounting 50%) to prepare ethno-medicines in Kani Tribes, India [39].

Whole plant and roots are other frequently used plant parts probably due to bioactive components enriched in these parts. However, their excessive use is detrimental for their survival since whole plant has to be uprooted. Not only roots, even the use of more than one plant part for medicinal purpose has put these plants to extinction risk owing to damage inflicted on the plants [40].

Mode of preparation

Herbal preparation is made by using different plant parts like whole plant, leaves, roots, stem, fruits, flowers, barks, berries and seeds.

Different modes of preparation are used for different plants by using various parts by the native people; this study showed 23.5% ethno-preparations were used in powdered form, 22.7% paste, 16.37% decoction, 12.62% juice, 8.75% poultice, and 8% each extract and infusion and 16.25% were not mentioned in literature.

Different modes of preparation of the plants are shown in Fig. 9.
Fig. 9
Fig. 9

Mode of preparation of plant

The missing information regarding mode of preparation of the ethno-medicine was also reported by Farnsworth who indicated the published studies include raw list of plants and only a small number of manuscripts reports the mode of preparation while the rest indicates the part used in treating a particular ailment [41].

Literature revealed that plants can be used in different ethno formulations depending upon the sort of skin disease to be treated and the type and part of plant selected to treat the disease. The ethno-formulation used for skin condition may include powder, paste, plant juice, ointment, poultice, extract, decoction or infusion [42, 43].

Decoction and infusion are frequently used in ethno-medicine due to the ease of preparation and because water is used as a solvent in these formulations, which is easily available [34]. Felhaber reported that ethno-medicines can be given orally or applied topically. Though both the routes can be used but topical application is common as well as effective as it ensures direct contact of active constituents of plant with the site of action (skin) and also because it gives a quick relief [44].

Combined ethno-preparation

Medicinal plants are used in combination with other medicinal plants, with vegetable oils (mustard oil, apricot oil etc.) and/or with the nutrients (milk, ghee etc.). People of Pakistan still rely on synergistic interactions of plants and supportive components like oil and milk to treat skin diseases.

These supplement ingredients may be used to enhance the effect of the herbal preparations or are simply used to make the preparations palatable. And on the other hand they improve the healing conditions; however, the exact role of these materials in curing the diseases is not clearly known [29].

A number of plant combinations used to treat skin conditions have been reported. The synergistic interaction of medicinal plants have been used since antiquity. Mabona and Van Vuuren reported that medicinal plants are used alone as well as in combination with other plants without any adequate validation of the combination. Plant species like Acorus calamus, Cyathula natalensis, Cyanella lutea, Hypoxis latifolia, Momordica foetida, Pittosporum viridiflorum and Vernonia natalensis have effects on skin when used in combination with other plants but if used alone, they do not have any dermatological effects; they may have potential to treat the additional symptoms associated with skin conditions like fever in skin infection [34].

Plants used in combination with other plants are given in Table 3, plants used in combination with different oils in Table 4 and plants used in combination with nutrients are given in Table 5.
Table 3

Combination of ethno plants used for skin conditions

Sr. no.


Mode of preperation

Folk claim



Cynodon dactylon (L.) + Curcuma longa L.

Grind along with Curcuma longa and rice

To stop bleeding from wounds, scabies, fungal infections, leprosy, wound healing, pimples, eczema, anti-septic, itching, small pox



Melia azedarach L. + Phyllanthus emblica L. + Terminalia chebula Retz.

Leaves are ground in water along with black pepper. Tablet are prepared from paste of dry friut of Melia azadarach, Phyllanthus emblica, and Terminalia chebula

Skin infection, wound healing and leprosy, scabies, pustules, pimples, boils and other skin diseases, allergy and itching, eczema and measles, boil, allergy, leucoderma, Cutaneous infections, Germicidal



Aloe vera (L.) + Citrus limon (L.) Burm. f.

Pulp of aloe along with 2–3 drops of lemon and rose extract is also used

Wound healing, chronic, dermatitis and radiation burn treatment, cuts, burns, eczema, sunburn, dermatitis, acne, freckles, pimples and boils, ulcer



Aloe barbadensis Mill. + Curcuma longa L. Aloe barbadensis Mill. + Cichorium intybus L.

Pulp of the leaf of Aloe is added with powdered Curcuma longa for dermal use on wounds. Pulp of the leaf of Aloe and chicory (Cichorium intybus) are soaked in water at night

Skin beauty, skin disease, wound healing, burn, eruption, boils, itchy skin



Cyperus rotundus L. + Curcuma longa L.

Roots of Cyperus rotundus L. along with turmeric and curd are made into a paste

leprosy, Skin diseases, pruritus, eczema, wound healing, pimples, acne



Xanthium stromarium Linn. + Calotropis procera (Aiton) R. Br

Fresh leaves of Xanthium stromarium Linn. are crushed with the leaves of Calotropis procera

leprosy, and purities, wound healing, Smallpox, eczema, Ulcers, boils



Canabis sativa Linn + Cassia fistula Schimp.ex.Oliv

The seed of Cassia fistula and one gram leaves of Cannabis sativa are taken along a cup of milk

Leprosy, Wound healing, Measles, Dressing of wounds and sores, Scabies



Clematis orientalis L. + PineWolf

The leaf paste used with resin of Pine on wounds to cure promptly

Antiseptic, pimples, antiseptic, burns, Eczema, Wound healing, Syphilis



Chenopodium album L. + Solanum surattense Burm. f.

Decoction of Chenopodium albumis is prepared with Solanum suttattense

Ulcer, Measles and other skin diseases



Fragaria nubicola (Hook. f.) nubicola (Hook. f.) + Berberis lyceum Royle

Powder of leaves and roots is mixed Berberis lycium

Skin infections


Table 4

Plants used in combination with oils

Botanical name

Common name


Part used

Ethno preparation

Folk claim


Acacia Arabica (Lam.) Willd.



F, L, S, Bk

mixed with oil And applied

Wound healing, eczema and other skin diseases


Aconitum heterophyllum Wall. ex Royle



R, Fl

The dried pulverized roots are mixed with butter oil

Abscess, boils and other skin diseases


adhatoda zeylanica Medik

Bhaikar, Barg-e-baansa and Arrusa



Ash of leaves is mixed with mustard oil and externally

Pustules and pimples


Allium cepa L.

Ghashoo, Tukhm peyaz, onion


S, L, bk, bu

One or two scales are warmed in mustard oil and crushed. This paste is applied twice a day for 2–3 days

Wound healing, Eruption, boils, Chicken pox, Pimples, Skin infections, gonorrhea and other skin diseases


Cleome viscosa Linn.

Kinni Buti


L, Sd

Leaves are coated with sesame oil and warmed over fire, which are applied over pain and inflammation of the boils

Boils, ringworm


Calotropis procera (Willd.) R. Br.

Dasi ak, Milk weed, Akk, Spalmaka, wallow-wort, Sodom apple, Dead Sea apple


S, Bk, L, Sd, R, Ft, WP

Latex is mixed with castor oil and applied on skin. Flowers are put in oil applied to wounds to cure them. Roots are powdered, mixed with “desi ghee” and pasted on points of leprosy

Leprosy, wound healing, abscess, ringworm, eczema, pustules and pimples, eruptions, syphilis, boils, ulcer, burn, dermatitis, scabies, skin infection


Lawsonia alba Lam.



L, Ft

Leaves are mixed with the mustard oil and made into paste

Leukoderma, athlete foot


Polygonum tataricum L.

Bro Kho-Bro


L, Sd

The flour is mixed with apricot oil in boiling water; paste is formed and applied to skin overnight.

Skin disorders


Potentilla nepalensis Hook.

Panzpatar, Rattenjot


L, R

The leaves are boiled in milk and applied/powder (ashes) is being applied with oil

Boil healing, burns and bruises


Sporobolus ioclados (Trin.)



Sd, L

Mixed with oil and applied



Bk, bark; Bu, bulb; F, fruit; Fl, flowers; Ft, fruit; In, inflorescence; L, leaf; Pd, pod; R, roots; S, stem; WP, whole plant

Table 5

Plants used in combination with nutrients

Botanical name

Common name


Part used


Folk claim


Ajuga bracteosa Wall. ex Benth

Khawaja booti,, Kauri Booti


L, R, WP

Macerate with yogurt is applied

Skin infections, Wound healing, pimples, measles, Boils


Zizipnus manurithiana Lam.




Leaves are poused into a paste mixed with gurh and then applied as a poultice



Bergenia ciliate A. Br. ex Engl.

Chata pana (Zakhm-e-hayat)


R, Fl, L, Bk, Sd, Rh

The powder of roots is mixed with Desi ghee

Wound healing, Sun burn, washing ulcers, infection, pimples and other skin diseases


Boerhaavia diffusa L.




Roots are crushed, boiled in milk and poultice is made



Citrus medica Linn.

Khatti, Naranj


L, Sd, Lt

Fruit extract is mixed with honey and fresh milk to make its paste

Itching, inflammation, Pimples


Convolvolus arvensis Linn

Heran Kari, Heran-paddi


P, Sh, L, F, R, S, Fl

Plant is ground along with black pepper and eaten

Wound healing, boils, scabies, ringworm


Cynoglossum denticulatum DC




Root is powdered and mixed with milk

Pustules and pimples


Ficus variegata Wall. Ex Roxb




Paste prepared from fresh milky juice of plant mixed milk

Boils, infected skin


Grewia tenax (Forssk.) Fiori

White spurry, Kaankeh, Wingo



The ash of the leaves is mixed with butter to make poultice

Abscess and wound healing, boils


Jurinea macrocephala Benth. ex Hook.f.

Gogol Doop



Decoction of roots is mixed with butter



Mallotus philippensis (Lam.) Müll. Arg.




Red powder of fruit is mixed with butter



Otostegia limbata (Benth.) Boiss

Kori booi, Spin Azghay, Chiti, Chittakanda, Ghawareja



Dried leaves are ground and mixed in butter to form a paste. Leaves are dried, ground and powder is mixed with honey. A table spoon is taken once a day

Wound healing and bruises


Pinus roxburghii Sarg

Chir, Nakhtar


AP, Sh, G, Bk, Rs

Young shoot is fried on pan and milk is added

Wounds, sores, burns, boils and ulcers, measles


Pinus wallichiana A. B. Jacks

Cheenh, Biar, Kail


Rs, W

Resin admixture with honey

Wound healing, antiseptic, Gonorrhea, abscess, for burning sensation, ulcer


Rumex nepalensis Spreng

Rawas, Hoola


L, S, R

Dried leaf powder is mixed with butter (ghee)

Wound healing and anti-allergy, boil


Stellaria media (Linn.)

Khashkhash boti, Losdhi, Salooni booti


WP, Sd, L

Seed powder with milk is given to children to cure skin infection and allergy

Wound healing, infections, allergy, itching-skin condition, eczema, ulcers, boils, abscesses, rashes, burns and irritations


Viola serpens Wall



WP, Fl

The whole plant is taken and boiled in milk till it become gelatinous. Bandage is made from it and used as poultice

Wound healing, eczema


Ac, milky acrid; AP, aerial parts; Bk, bark; Bu, bulb; F, fruit; Fl, flowers; Ft, fruit; G, gum; In, inflorescence; L, leaf; Lt, latex; P, Petals; Pd, pod; R, roots; Rh, rhizomes; Rs, resin; S, stem; Sh, shoots; W, wood; WP, whole plant

Endangered species

Endangered medicinal plants of Pakistan that have got their role in treating skin conditions are discussed in Table 6.
Table 6

Endangered species of Pakistan

Sr. no

Botanical name



Major threat

Conservation action



Aconitum chasmanthum Stapf ex Holmes



I. Over harvesting and loss of habitat due to construction of high altitude roads and avalanch

II. Regeneration of the species is hampered due to unsustainable collection of tubers and roots

I. Intensive studies on the population trend, reproductive biology and propagation techniques should be carried out to support conservation action programs

II. Habitat managment and sustainable collection practice

III. Active in situ conservation may be undertaken in protected areas

IV.Surveying and monitoring is needed across the known range of occurrence to ascertain the status of wild subpopulations (assessed on 25.12.2016)


Aconitum heterophyllum Wall. ex Royle



I. Loss of habitat due to road construction and unsustainable collection from wild

II. Large scale collection

III. This species is under severe threat due to illegal collection and marketing

I. Intensive studies on the population trend, reproductive biology and propagation techniques should be carried out to support conservation action programs

II. Habitat managment and sustainable collection practice

III. Active in situ conservation may be undertaken in protected areas

IV. Surveying and monitoring is needed across the known range of occurrence to ascertain the status of wild subpopulations (assessed on 25.12.2016)


Aconitum violaceum Jacquem. ex Stapf



I. Loss of habitat due to agriculture and unsustainable

II. Collection from wild

I. Habitat loss and over exploitation

II. It needs immediate attention in habitat management and sustainable collection practices

III. Active in situ conservation should be undertaken in protected areas

IV. Surveying and monitoring is also needed throughout the known historic range of the taxon to ascertain the status of all recorded subpopulations

V. Intensive studies on population trend, reproductive biology and propagation techniques need to be carried out to support conservation action programs (assessed on 25.12.2016)


Betula utilis D. Don



I. Over exploitation as it is a high value medicinal plant

II. In the Mankial Valley Hindukush Range, Pakistan, 85% of the population has decreased

Harvesting must be sustainable to ensure the survival of this species (assessed on 25.12.2016)


Cedrus deodara (Roxb. ex D. Don) G. Don



I. Intensive logging (legal and illegal)

II. Deforestation and conversion of forests for agriculture may also pose local threats in some parts of Pakistan and India

Known from several protected areas across its range (assessed on 25.12.2016)


Commiphora wightii (Arn.) Bhandari



I. Unsustainable collection of multiple parts, high volume trade and loss of habitat

II. Grazing and browsing by sheep and goats

III. Collection of branches as fuel wood during the rainy season, scarcity or festive times

IV. This species demonstrates one of the most generic problems of conservation

V. Overexploitation, a narrow extent of occurrence, small area of occupancy, severe fragmentation of populations, very low regeneration and invasion of alien species mean that C. wightii is facing a high extinction risk

I. Biotic pressure should be regulated

II. Standard and better gum extraction technique could minimize the mortality rate of the species (Dixit and Rao 2000)

III. Ex situ conservation and multiplication through micro and macro propagation technique

IV. Some attention and efforts have been brought into the system by identifying and documenting more than 100 forest areas (MPCAs) (assessed on 25.12.2016)


Ephedra intermedia

Schrenk ex C.A. Mey.



Over harvesting should be investigated

Monitoring of wild harvesting is recommended to better understand how this is affecting population size (assessed on 25.12.2016)


Gentiana kurroo Royle



I. Loss of habitat and unregulated harvesting. Due to road construction and agricultural invasion

II. Over grazing and human settlements

III. Climate change, in terms of temperature and rainfall, has severe impacts on the population and habitat

I. It needs immediate attention in terms of trade regulation, habitat management and sustainable collection practice

II. Ex situ conservation and cultivation may help to reduce the pressure on wild population (assessed on 25.12.2016)


Juniperus excels M. Bieb.



No specific range wide threats have been identified for this species; over exploitation and habitat degradation and conversion may be localised problems

This species is known from several protected areas throughout its range (assessed on 25.12.2016)


Juniperus squamata Lamb.




This species is recorded from many protected areas (assessed on 25.12.2016)


Pinus gerardiana Wall. Ex D. Don



I. Conversion for pine forests for agricultural use, increasing the degree of fragmentation, and overgrazing that prevents natural regeneration

II. Over harvesting of seed cones contributes to poor regeneration

III. Over exploitation for firewood

I. In Afghanistan, plantations have been established to supply the seeds

II. In other parts of its range P. gerardiana forests are included within protected areas

III. A combination of reafforestation programmes coupled with sustainable use strategies are needed before this species declines sufficiently to become eligible for a threatened category (assessed on 25.12.2016)


Pinus roxburghii Sarg.



While forest destruction and logging have reduced the area of occupancy (AOO) of P. roxburghii, it is still covering extensive areas (an estimated 0.87 million ha in India alone) and is therefore not considered to be threatened with extinction. Improved methods of resin tapping have decreased the risk of trees dying prematurely

This species occurs in some protected areas (assessed on 25.12.2016)


Pinus wallichiana A.B. Jacks.



Potentially, over-exploitation could negatively impact the population, but the species is too common and wide-spread for this to have serious consequences other than locally

This species occurs in several protected areas (assessed on 25.12.2016)

CR, critically endangered; EN, endangered; LC, least concern; NT, near threat; VU, vulnerable

Using the part like roots, rhizomes or bulbs could be a severe threat for reproduction of medicinal plants of the area. The plants collected by using these methods, especially those propagated through rhizome, bulb or corm, need sustainable utilization and conservation strategies. Un-sustainability of harvesting of herbaceous roots is well recognized by conservationists and termed such medicinal plants as highly threatened [40]. Uprooting a plants is the most detrimental method of plant collection, if the roots are not removed completely it can also result in destruction by decreasing water upset and increasing susceptibility of fungal infection. Commercialization is the major cause of extinction of medicinal plants in South Africa which demands over harvesting and thus has taken natural medicinal resources to near extinction [45].

Shanwari suggested to establish protocols to interpret the pattern of plant growth and to accelerate the knowledge about propagation of medicinal plants and avoiding harvesting of wild species in order to save endangered species [15].

Aconitum chasmanthus, A. heterophyllumand, A. violaceum, which are native to Pakistan, India and Nepal [4648], are harvested for their tubers which constitute Ayurveda drug and for this reason whole plants are uprooted. In 2003 in a CAMP workshop organized at India (Shimla), experts agreed that 80% of the wild population of Aconitum chasmanthus had declined and therefore it is assessed as “critically endangered species”, 70% of that of A. heterophyllum had declined and it is assessed as “endangered” while 40% decline of the population of A. violaceum has made it a “vulnerable” specie to become endangered. This decline is over a decade but the situation has not improved and therefore the status id still valid [49]. It was observed in a survey in India that collection amount of A. heterophyllum has dropped down from 200 g per person per day to 70–100 g per person per day in 5 years [50]. Moreover, illegal collection of this specie has let it threatened [51].

Reddy et al. reported that oleo-gum resin tapped from the stems of Commiphora wightii constitutes the well-known Ayurvedic drug “Guggul” which is consumed in high volumes by the herbal industries of Sub-continent. Field observations over the last several decades have confirmed a severe decline in its wild population, as the shrubs tapped for oleo-gum resin die within 2–3 years. Over the past 84 years (three generation lengths) there has been a decline of more than 80% in the wild population as a result of habitat loss and degradation, coupled with unregulated harvesting and tapping of oleo-gum resin. This species is therefore assessed as Critically Endangered [52]. Soni identified a number of relevant activities within the study area under the theme ‘Guggal Bachao Abhiyan’ (Save Guggal Movement). These were conducted through the close co-operation of the village level communities, who depend on local biodiversity for their livelihoods in the Aravali Hills of Rajasthan [53].

Gentiana kurroo is mainly collected from the wild and there is no information regarding its cultivation. Hence, the species is under severe threat of extinction. This assessment was primarily based on the very limited reported presence of the species in wild and the high demand and prices for the dried roots of this plant. The wild population in IndoPak region is inferred to have declined by 80% in a 10 years time period. The recent CAMP assessment also agrees with the trend of population decline of more than 80% in India. Therefore, the species is assessed as Critically Endangered [54, 55].

Commercially available important plants

Plants that are available commercially in Pakistan in different formulations are enlisted in Table 7.
Table 7

List of some commercially available medicinal plants used in Pakistan

Sr. no

Botanical name

Brand name



Dosage form



Calotropis procera (Willd.) R. Br.


Hamdard laboratories Waqf Pakistan

Ammonium chloride68.070000 mg/Tab

Calotropis procera (Ait.) R. Br.11.345000 mg/Tab

Ferula assafoetida Linn.5.627000 mg/Tab

Lake salt68.070000 mg/Tab

Myrtus caryophyllus Spreng. 84.952000 mg/Tab

Piper nigrum Linn.34.035000 mg/Tab

Potassium Carbonate11.345000 mg/Tab

Sanchal Salt84.952000 mg/Tab

Sodii biboras11.345000 mg/Tab

Zingiber officinale Roscoe 34.035000 mg/Tab


Twice a day


Berberis lyceum Royle



Ammonium chloride 30.000000 mg/10 ml

Berberis aristata DC. 200.000000 mg/10 ml

Cichorium intybus Linn. 100.000000 mg/10 ml

Citrus limonum Risso (Oil) 10.000000 mg/10 ml

Citrus limonum Risso (Bark) 300.000000 mg/10 ml

Ferrous ammonium sulphate 50.000000 mg/10 ml

Jawahar mohra 10.000000 mg/10 ml

Nelumbium speciosum Willd. 100.000000 mg/10 ml

Rosa damascena Miller 100.000000 mg/10 ml

Santalum album Linn. 26.660000 mg/10 ml

Strychnos nux-vomica Linn. (Extract) 10.000000 mg/10 ml

Vitis vinifera Linn. 200.000000 mg/10 ml

Preservatives 0.000000 Q.S

Saccharum Base 0.000000 Q.S


Twice a day


Achyranthes aspera L.



Achyranthes aspera Linn.312.000000 mg/10 ml

Adhatoda vasica Nees125.000000 mg/10 ml

Ephedra gerardiana Wall. ex Stapf125.000000 mg/10 ml

Glycyrrhiza glabra Linn.125.000000 mg/10 ml

Khashkhash musaffa125.000000 mg/10 ml

Mentha piperita Linn. (Extract) 2.500000 mg/10 ml

Pistacia integerrima J. L. Stewart ex Brandis125.000000 mg/10 ml


6 times a day


Riccinus communis L.


Hamdard laboratories Waqf Pakistan

Celastrus paniculatus Willd. (Oil) 0.500000 g/3 g

Cinnamomum cassia Blume (Oil) 0.100000 g/3 g

Ricinus communis Linn. (Oil) 1.500000 g/3 g

Sea Salt0.320000 g/3 g

Styrax benzoin Dryander 0.160000 g/3 g

Wax0.320000 g/3 g


3 g once daily


Carthamus tinctorious L.


Hamdard laboratories Waqf Pakistan

Ammonium chloride 15.500000 mg/500 mg,

Black salt 15.500000 mg/500 mg

Carthamus tinctorius Linn. 15.500000 mg/500 mg

Carum carvi Linn. 15.500000 mg/500 mg

Cinnamomum cassia Blume 15.500000 mg/500 mg

Cinnamomum malabathrum Batka 15.500000 mg/500 mg

Cuminum cyminum Linn. 15.500000 mg/500 mg

Cuscuta reflexa Roxb. 15.500000 mg/500 mg

Foeniculum vulgare Miller 15.500000 mg/500 mg

Lake salt 30.750000 mg/500 mg

Piper nigrum Linn. (White) 15.500000 mg/500 mg

Piper nigrum Linn. (Black) 15.500000 mg/500 mg

Rock Salt 185.000000 mg/500 mg

Valeriana officinalis Linn. 15.500000 mg/500 mg

Zingiber officinale Roscoe 15.500000 mg/500 mg


2 tab once daily


Solanum nigrum L.



Acacia arabica (Lam.) Willd. 62.500000 mg/10 ml

Achillea millefolium Linn. 62.500000 mg/10 ml

Adiantum capillus-veneris Linn 62.500000 mg/10 ml

Cichorium endivia Linn. 62.500000 mg/10 ml

Ficus bengalensis Linn. 62.500000 mg/10 ml

Fumaria offcinalis 62.500000 mg/10 ml

Gendarussa vulgaris 62.500000 mg/10 ml

Iron Compound 50.000000 mg/10 ml

Juniperus communis Linn. var. saxatilis Pall. 62.500000 mg/10 ml

Melia azadarach Linn. 62.500000 mg/10 ml

Nepeta ruderalis Ham. 62.500000 mg/10 ml

Pimpinella anisum Linn. 62.500000 mg/10 ml

Rubia cordifolia Linn. 62.500000 mg/10 ml

Solanum nigrum Linn. 62.500000 mg/10 ml


2 teaspoon twice a day


Aloe barbadensis Mill.


Hamdard laboratories Waqf Pakistan

Aloe barbadensis Mill. 1.000000 g/100 ml


5 teaspoon thrice a day


Ficus carica Linn.


Hamdard laboratories Waqf Pakistan

Acacia arabica (Lam.) Willd. 23.520000 mg/Tab

Ficus caricaLinn. 23.520000 mg/Tab

Melia azadarach Linn. (Peel) 23.520000 mg/Tab

Melia azadirachta Linn (Leaves) 23.520000 mg/Tab

Melia azadirachta Linn (Bark) 23.520000 mg/Tab

Psoralea corylifolia Linn. 23.520000 mg/Tab


3 tab thrice a day


Fumaria indica Linn


Hamdard laboratories Waqf Pakistan

Cassia angustifolia Vahl. 0.075000 g/6 g

Emblica officinalis Linn. (Dry) 0.150000 g/6 g

Fumaria indica (Haussk) Pugsley (Leaves) 0.375000 g/6 g

Rosa damascene Miller 0.045000 g/6 g

Terminalia chebula Retz. 0.150000 g/6 g

Sweetening agent and preservatives 0.000000 Q.S

Semi solid

6–12 g daily


Datura stramonium L.


Hamdard laboratories Waqf Pakistan

Asphaltum 3.330000 g/Tab

Bombax ceiba Linn. 16.660000 g/Tab

Centaurea behenLinn. 8.330000 g/Tab

Corylus avellana Linn. 2.660000 g/Tab

Datura stramonium Linn. 2.660000 g/Tab

Hyoscyamus niger Linn. 2.660000 g/Tab

Juglans regia Linn. 2.660000 g/Tab

Lactuca scariola Linn. 2.660000 g/Tab

Lagenaria vulgaris Ser 2.660000 g/Tab

Myristica fragrans Houtt. (Seeds (nutmeg)) 1.330000 g/Tab

Myristica fragrans Houtt. (Seeds (mace)) 1.330000 g/Tab

Papaver somniferum Linn. 2.660000 g/Tab

Pinus gerardiana Wall ex Lam. 2.660000 g/Tab

Pistacia vera Linn. 2.660000 g/Tab

Prunus amygdalus Batsch. 2.660000 g/Tab


1–2 tab once daily

Worth and number of herbal industries is increasing day by day in Pakistan due to the trust of people on traditional medicines [56].

Ethno-medicines has a vital role in the industrialization [57]. Vivienne et al. stated commercial importance of medicinal plants in South Africa and reported 11 species of medicinal plants that are imported from India and other countries including Cinnamommum camphora. They also concluded that the size of the regional market of medicinal plants can be assessed by knowing the number of species traded. Commercial utilization of the medicinal plant is directly related to the degree of extinction [45].

Future considerations

The review revealed that many of the important information like ethno preparation, habit and part used, of many important medicinal plants were not available in previous articles which can be due to the lack of interest of local youth to acquire the traditional knowledge from the ancestors and thus provision of incomplete information to the previous articles, further ethno-pharmacological research should be carried out to save the traditional knowledge and to take it to the light of science.

Medicinal plants in Pakistan contain great variety which can be used against a large number of skin ailments. Leaves, whole plant and roots are the most widely used parts in different ethno-medicinal preparations. Whole plant and root harvesting are the destructive type of techniques, it is important to protect the medicinal plants from exploitation. Although in Pakistan there is a strong traditional background supporting the use of these ethno-medicines against skin ailments but detailed ethno-pharmacological studies are not enough to support the folk claim. Majority of the studies have not documented other information regarding mode of preparation and dose of ethno-medicine. Therefore it is necessary to carry out a comprehensive study on ethno-pharmacology in Pakistan. It is important as the folk knowledge supplements a scientific investigations and penetrations with the primary information. Very limited number of studies provides toxic profile of these medicinal plants, toxicity studies should be carried out for these medicinal plants in animal system to establish a safe dose range.

More pharmacological studies (in vitro and in vivo) should be carried out on medicinal plants of Pakistan that are relatively unexplored or less explored. Mostly, the extracts were tested against different pathogens, for wound healing properties and for other skin conditions. However, very few classes of secondary metabolites and pure isolated components were tested. Therefore, it is imperative to conduct detailed phytochemical studies for the isolation of novel compounds.


Authors’ contributions

A gave basic concept of the paper and reviewed and drafted the manuscript, AS, HA, WY, HR, also collected and analyzed data and drafted the manuscript. IAB and AMA collected papers using facilities of their university and also helped in writing paper. All authors read and approved the final manuscript.


The authors extend their appreciation to the deanship scientific research at King Saud university for funding this research through # (RG-1439-002).

Competing interests

The authors declare that they have no competing interests.

Availability of data and materials

All data generated and analyzed in this study is has been taken from the free excess sources while those which were not available online were taken by authors from king Saud University.

Consent for publication

Not applicable.

Ethics approval and consent to participate

Not applicable.


Not applicable.

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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

Laboratory of Cardiovascular Research and Integrative Pharmacology, College of Pharmacy, University of Sargodha, Sargodha, Pakistan
Rashid Latif College of Pharmacy, Lahore, Pakistan
Department of Pharmacology, College of Medicine, King Saud University Riyadh, Riyadh, Saudi Arabia
Prince Abdullah Ben Khaled Celiac Disease Research Chair, Department of Pediatrics, Faculty of Medicine, King Saud University, Riyadh, Saudi Arabia


  1. Musharaf K. Ethnobotanical studies on plant resources of Sheikh Maltoon, District Mardan, Pakistan. J Med Plants Res. 2014;4(5):35–45.Google Scholar
  2. Maroyi A. Traditional use of medicinal plants in south-central Zimbabwe : review and perspectives. J Ethnobiol Ethnomed. 2013;9:31.PubMed CentralView ArticlePubMedGoogle Scholar
  3. Ahmad M, Sultana S, Fazl-I-Hadi S, Ben Hadda T, Rashid S, Zafar M, Khan MA, Khan M, Yaseen G. An ethnobotanical study of medicinal plants in high mountainous region of Chail valley (District Swat–Pakistan). J Ethnibiol Ethnomed. 2014;10(1):36.View ArticleGoogle Scholar
  4. Abbasi AM, Khan MA, Shah MH, Shah MM, Pervez A, Ahmad M. Ethnobotanical appraisal and cultural values of medicinally important wild edible vegetables of Lesser Himalayas-Pakistan. J Ethnibiol Ethnomed. 2013;9(1):66.View ArticleGoogle Scholar
  5. Bin Asad HHM, Murtaza G, Siraj S, Khan AS, Azhar S, Hussain SM, Ismail T, Hussain SM, Hussain I. Enlisting the scientifically unnoticed medicinal plants of Pakistan as a source of novel therapeutic agents showing anti-venom activity. Afr J Pharm Pharmacol. 2011;5(20):2292–305.Google Scholar
  6. Mussarat S, Abdel-Salam NM, Tariq A, Wazir SM, Ullah R, Adnan M. Use of ethnomedicinal plants by the people living around indus river. Evid Based Complement Alternat Med. 2014;2014:212634.PubMed CentralView ArticlePubMedGoogle Scholar
  7. Khan AS, Hassan M, Ali S. Secondary metabolite studies of some selected plants of District Gilgit, Gilgit-Baltistan. Int J Pharmacog Phytochem Res. 2014;6(3):467–71.Google Scholar
  8. Ahmad SS, Erum S, Khan MS, Nawaz M, Abdul W. Exploring the medicinal plants wealth: a traditional medico-botanical knowledge of local communities in Changa Manga Forest, Pakistan. Middle-East J. 2014;20(12):1772–9.Google Scholar
  9. Manzoor M, Durrani J, Ayesha A. Uses of fruits, vegetables and herbs for the treatment of diabetes by the people of Quetta city. Sci Tech Dev. 2013;32(1):24–7.Google Scholar
  10. Johnsy G, Sargunam SD, Kaviyarasan V. Indigenous knowledge of medicinal plants used for the treatment of skin diseases by the Kaani tribe of Kanyakumari District. Int Pharm Pharmaceut Sci. 2012;4(1):309–13.Google Scholar
  11. Ali H, Qaiser M. The ethnobotany of chitral valley. Pakistan with particular reference to medicinal plants. Pak J Bot. 2009;41(4):2009–41.Google Scholar
  12. Bibi T, Ahmad M, Tareen BR, Tareen MN, Jabeen R, Ur-Rehman S, Sultana S, Zafar M, Yaseen G. Ethnobotany of medicinal plants in district Mastung of Balochistan province-Pakistan. J Ethnopharmacol. 2014;157:79–89.View ArticlePubMedPubMed CentralGoogle Scholar
  13. Arshad M, Ahmad M, Ahmed E, Saboor A, Abbas A, Sadiq S. An ethnobiological study in Kala Chitta hills of Pothwar region. Pakistan: multinomial logit specification. J Ethnobiol Ethnomed. 2014;10:13.PubMed CentralView ArticlePubMedGoogle Scholar
  14. Gilani SA, Khan AM, Qureshi RA, Sherwani SK, Rehman RU, Bukhari TZ. Advances in bioresearch ethno-medicinal treatment of common gastrointestinal disorders by indigenous people in Pakistan. Adv Biores. 2014;5(1):42–9.Google Scholar
  15. Shinwari KZ. Medicinal plants research in Pakistan. J Med Plants Res. 2010;4(3):161–76.Google Scholar
  16. Ahmed JM, Malik HZ, Khan S, Nasar S. Biological spectrum and ethnomedicinal uses of plants in Chellah district Muzaffarabad Azad Kashmir Pakistan. J Adv Bot Zool. 2014;1(4):1–5.Google Scholar
  17. Shedayi AA, Gulshan B. Ethnomedicinal uses of plant resources in Gilgit-Baltistan of Pakistan. J Med Plants Res. 2012;6(29):4540–9.Google Scholar
  18. Shaheen H, Nazir J, Firdous SS, Khalid A. Cosmetic ethnobotany practiced by tribal women of Kashmir Himalayas. Avicenna J Phytomed. 2014;4(4):239–50.PubMed CentralPubMedGoogle Scholar
  19. Hussain S, Malik F, Riaz H, Qayyum MA, Khalid N. Alternative and traditional medicines systems in Pakistan: history, regulation, trends, usefulness, challenges, prospects and limitations. New York: INTECH Open Access Publisher; 2012.Google Scholar
  20. Baloch PM, Marri MY, Qaisrani MA, Baloch A. Plants treasures, traditional knowledge and baloch society. Bi Ann Res J. 2013;28(1):1810–2174.Google Scholar
  21. Bano A, Ahmad M, Zafar M, Sultana S, Rashid S, Khan MA. Ethnomedicinal knowledge of the most commonly used plants from Deosai Plateau, Western Himalayas, Gilgit Baltistan, Pakistan. J Ethnopharmacol. 2014;155:1046–52.View ArticleGoogle Scholar
  22. Ahmad SK, Habib S. Indigenous knowledge of some medicinal plants of himalaya region, Dawarian Village, Neelum Valley, Azad Jammu and Kashmir, Pakistan. Univ J Plant Sci. 2014;2(2):40–7.Google Scholar
  23. Hostettmann K, Marston A, Ndjoko Loset K, Wolfender JL. The potential of African plants as a source of drugs. Curr Org Chem. 2000;4(10):973–1010.View ArticleGoogle Scholar
  24. Dawid-Pać R. Medicinal plants used in treatment of inflammatory skin diseases. Postepy Dermatol Alergol. 2013;30(3):170–7.PubMed CentralView ArticlePubMedGoogle Scholar
  25. De Wet H, Nkwanyana MN, van Vuuren SF. Medicinal plants used for the treatment of diarrhoea in northern Maputaland, KwaZulu-Natal Province, South Africa. J Ethnopharmacol. 2010;130(2):284–9.View ArticleGoogle Scholar
  26. Njoroge GN, Bussmann RW. Ethnotherapeautic management of skin diseases among the Kikuyus of Central Kenya. J Ethnopharmacol. 2007;111(2):303–7.View ArticleGoogle Scholar
  27. Doğan A, Demirci S, Cağlayan AB, Kılıç E, Günal MY, Uslu U, Cumbul A, Sahin F. Sodium pentaborate pentahydrate and pluronic containing hydrogel increases cutaneous wound healing in vitro and in vivo. Biol Trace Elem Res. 2014;7(3):37–44.Google Scholar
  28. Tabassum N, Hamdani M. Plants used to treat skin diseases. Pharmacogn rev. 2014;8(15):52.PubMed CentralView ArticlePubMedGoogle Scholar
  29. Tariq A, Musarat S, Adnan M. Review on ethno medicinal, phytochemical and pharmacological evidence of Himalayan anticancer plants. J Ethnopharmacol. 2015;164:96–119.View ArticlePubMedPubMed CentralGoogle Scholar
  30. Kumar K, Sharma YP, Manhas RK, Bhatia H. Ethnomedicinal plants of Shankaracharya Hill, Srinagar, J&K, India. J Ethnopharmacol. 2015;170:255–74.View ArticlePubMedPubMed CentralGoogle Scholar
  31. Barker MS, Kane NC, Matvienko M, Kozik A, Michelmore RW, Knapp SJ, Rieseberg LH. Multiple paleopolyploidizations during the evolution of the Compositae reveal parallel patterns of duplicate gene retention after millions of years. Mol Biol Evol. 2008;25(11):2445–55.PubMed CentralView ArticlePubMedGoogle Scholar
  32. Kumar S, Malhotra R, Kumar D. Euphorbia hirta: its chemistry, traditional and medicinal uses, and pharmacological activities. Pharmacogn Rev. 2010;4(7):58–61.PubMed CentralView ArticlePubMedGoogle Scholar
  33. Saikia AP, Ryakala VK, Sharma P, Goswami P, Bora U. Ethnobotany of medicinal plants used by Assamese people for various skin ailments and cosmetics. J Ethnopharmacol. 2006;106(2):149–57.View ArticlePubMedPubMed CentralGoogle Scholar
  34. Mabona U, Van Vuuren FS. Southern African medicinal plants used to treat skin diseases. S Afr J Bot. 2013;87:175–93.View ArticleGoogle Scholar
  35. Khan SM, Page S, Ahmad H, Harper D. Identifying plant species and communities across environmental gradients in the Western Himalayas: method development and conservation use. Ecol Inf. 2013;14:99–103.View ArticleGoogle Scholar
  36. Ahmad M, Qureshi R, Arshad M, Khan MA, Zafar M. Traditional herbal remedies used for the treatment of diabetes from district Attock (Pakistan). Pak J Bot. 2009;41(6):2777–82.Google Scholar
  37. Bhat JA, Kumar M, Bussmann RW. Ecological status and traditional knowledge of medicinal plants in Kedarnath Wildlife Sanctuary of Garhwal Himalaya, India. J Ethnobiol Ethnomed. 2013;9:1.PubMed CentralView ArticlePubMedGoogle Scholar
  38. Ghorbani A. Studies on pharmaceutical ethnobotany in the region of Turkmen Sahra, north of Iran:(part 1): general results. J Ethnopharmacol. 2005;102(1):58–68.View ArticlePubMedPubMed CentralGoogle Scholar
  39. Ayyanar M, Ignacimuthu S. Ethnobotanical survey of medicinal plants commonly used by Kani tribals in Tirunelveli hills of Western Ghats, India. J Ethnopharmacol. 2011;134:851–64.View ArticlePubMedPubMed CentralGoogle Scholar
  40. Adnan M, Ullah I, Tariq A, Murad W, Azizullah A, Khan AL, Ali N. Ethnomedicine use in the war affected region of northwest Pakistan. J Ethnobiol Ethnomed. 2014;10:1–16.View ArticleGoogle Scholar
  41. Farnsworth NR. The role of ethnopharmacology in drug development. In: Chadwick DJ, Marsh J, editors. Bioactive compounds from plants Ciba-Geigy Symposium No154 Bangkok 20–22February. Wiley: New York; 1990. p. 2–21.Google Scholar
  42. Watt JM, Breyer-Brandwijk MG. The medicinal and poisonous plants of southern and eastern Africa. 2nd ed. London: Livingstone Edinburgh; 1962. p. 205–6.Google Scholar
  43. Hutchings A. Zulu medicinal plants: an inventory. Kwazulu: University of Kwazulu Natal Press; 1996.Google Scholar
  44. Felhaber T. South African traditional healers’ primary health care handbook, Kagiso Publishers. South Africa: Cape Town; 1997.Google Scholar
  45. Williams VL, Balkwill K, Witkowski ET. Unraveling the commercial market for medicinal plants and plant parts on the Witwatersrand, South Africa. Econ Bot. 2000;54(3):310–27.View ArticleGoogle Scholar
  46. Bhatt D, Joshi GC, Kumar R, Tewari LM. Phytosociological features and threat categorization of A heterophyllum Wall. ex. Royle and A. ferox Wall. ex. Ser. in Kumaun. J Ecol Nat Environ. 2014;6(3):111–8.View ArticleGoogle Scholar
  47. Ved DK, Kinhal GA, Ravikumar K, Prabhakaran V, Ghate U, Sankar RV, Indresha JH. Conservation assessment & management prioritisation for the medicinal plants of Jammu & Kashmir. Himachal Pradesh: Foundation for Revitalisation of Local Health Traditions; 2003.Google Scholar
  48. Ved DK, Kinhal GA, Ravikumar K, Sankar RV, Haridasan K. Conservation Assessment and Management Prioritisation (CAMP) for wild medicinal plants of North-East India. Med Plant Conserv. 2005;11:40–4.Google Scholar
  49. Belt J, Lengkeek A, VanderZant J. Cultivating a healthy enterprise: developing a sustainable medicinal plant chain in Uttaranchal-India. Amsterdam: KIT Publishers; 2003. p. 350.Google Scholar
  50. Srivastava N, Sharma V, Kamal B, Jadon VS. Aconitum: need for sustainable exploitation (with special reference to Uttarakhand). Int J Green Pharm. 2010;4:220–8.View ArticleGoogle Scholar
  51. Soni V. Conservation of Commiphora wightii, an endangered medicinal shrub, through propagation and planting, and education awareness programs in the Aravali Hills of Rajasthan, India. Cons Eviron. 2010;7(2):27–31.Google Scholar
  52. Goraya GS, Jishtu V, Rawat GS, Ved DK. Wild medicinal plants of Himachal Pradesh: an assessment of their conservation status and management prioritisation. Shimla: Himachal Pradesh Forest Department; 2013.Google Scholar
  53. Singh SK, Rawat GS. Flora of Great Himalayan National Park: Himachal Pradesh; 2000.Google Scholar
  54. Shinwari ZK, Watanabe T, Rehman M, Youshikawa T. A Pictorial Guide to medicinal plants of Pakistan. Kohat: Kohat University of Science and Technology; 2006.Google Scholar
  55. Cano HJ, Volpado G. Herbal mixtures in the traditional medicines of eastern Cuba. J Ethnopharmacol. 2004;90:293–316.View ArticlePubMedPubMed CentralGoogle Scholar
  56. Haq F, Ahmad H, Alam M. Traditional uses of medicinal plants of Nandiar Khuwarr catchment (District Battagram), Pakistan. J Med Plants Res. 2011;5(1):39–48.Google Scholar
  57. Arshad M, Nisar FM, Majeed A, Ismail S, Ahmad M. Ethno medicinal flora in District Sialkot, Punjab, Pakistan. Middle-East J Sci Res. 2011;9(2):209–14.Google Scholar
  58. Kamal M, Wazir SM, Hassan M, Subhan M, Khan SU, Muhammad M, Taj S. Ethnobotanically important plants of district Bannu, Pakistan. Pak J Plant Sci. 2009;15(2):87–93.Google Scholar
  59. Qureshi RM, Maqsood M, Arshad M, Chaudhry AK. Ethnomedicinal uses of plants by the people of Kadhi areas of Khushab, Punjab, Pakistan. Pak J Bot. 2011;43(1):121–33.Google Scholar
  60. Ishtiaq M, Mumtaz SA, Hussain T, Ghani A. Medicinal plant diversity in the flora of Leepa Valley, Muzaffarabad (AJK), Pakistan. Afr J Biotechnol. 2012;11(13):3087–98.Google Scholar
  61. Imtiaz S, Abdullah S, Afzal S, Rehman G, Waheed M. Medicinal plants used by traditional healers of Punjab, Pakistan. Can J App Sci. 2013;3(4):496–515.Google Scholar
  62. Begum S, Abdeislam NM, Adnan M, Tariq A, Yasmin A, Hameed R. Ethnomedicines of highly utilized plants in the temperate himalayan region. Afr J Tradit Complement Altern Med. 2014;11(3):132–42.PubMed CentralView ArticlePubMedGoogle Scholar
  63. Mahmood A, Mahmood A, Mujtaba G, Mumtaz MS, Kayani WK, Khan MA. Indigenous medicinal knowledge of common plants from district Kotli Azad Jammu and Kashmir Pakistan. J Med Plant Res. 2012;6:4961–7.View ArticleGoogle Scholar
  64. Abbasi AM, Khan MA, Ahmed M, Zafar M. Herbal medicines used to cure various ailments by the inhabitants of Abbottabad district, North West Frontier Province, Pakistan. Indian J Trad Know. 2010;9(1):175–83.Google Scholar
  65. Sabeen M, Ahmad DSS. Exploring the folk medicinal flora of Abbotabad City, Pakistan. Ethnobotl Leaflets. 2009;13:810–33.Google Scholar
  66. Rauf F, Qureshi R, Shaheen H. Folk medicinal uses of indigenous plant species of Barroha, Bhara Kahu and Maanga in Islamabad, Pakistan. J Med Plant Res. 2012;6(11):2061–70.Google Scholar
  67. Hussain K, Nisar FM, Majeed A, Nawaz K, Bhatti HK. Ethnomedicinal survey for important plants of Jalalpur Jattan, District Gujrat, Punjab, Pakistan. Ethnobot Leaflets. 2010;14:807–25.Google Scholar
  68. Naz I, Ahmad M, Ul-Hassan T. Ethnobotanical investigation of medicinal flora used by indigenous People in District Attock, Pakistan. J Adv Bot Zool. 2014;1(4):1–7.Google Scholar
  69. Ahmad M, Khan AM, Manzoor S, Zafar M, Sultana S. Check list of medicinal flora of Tehsil Isakhel, District Mianwali-Pakistan. Ethnobot Leaflets. 2006;10:41–8.Google Scholar
  70. Ghani A, Ali Z, Perveen S. Folk recipes and ethno botanical survey of medicinal plants Mianwali district (Pakistan). Int J Curr Pharm Res. 2012;4(2):61–3.Google Scholar
  71. Panhwar QA, Abro H. Ethnobotanical studies of Mahal Kohistan (Khirthar national park). Pak J Bot. 2009;39(7):2301–15.Google Scholar
  72. Mahmood A, Mahmood A, Shaheen H, Qureshi RA, Sangi Y, Gilani SA. Ethno medicinal survey of plants from district Bhimber Azad Jammu and Kashmir, Pakistan. J Med Plant Res. 2011;5(11):2348–60.Google Scholar
  73. Jabeen A, Khan MA, Ahmad M, Zafar M, Ahmad F. Indigenous uses of economically important flora of Margallah hills national park, Islamabad, Pakistan. Afr J Biotechnol. 2009;8(5):763–84.Google Scholar
  74. Mahmood A, Mahmood A, Malik RN. Indigenous knowledge of medicinal plants from Leepa valley, Azad Jammu and Kashmir, Pakistan. J Ethnopharmacol. 2012;143(1):338–46.View ArticlePubMedPubMed CentralGoogle Scholar
  75. Nisar MF, Jaleel F, Haider SM, Toor Y, Ismail S, Arfan M, Azeem M. Exploration of ethno-medicinal plants and their ritual uses in Bahawalnagar, Pakistan. Middle-East J Sci Res. 2014;21(9):1466–71.Google Scholar
  76. Shedayi AA, Xu M, Gulraiz B. Traditional medicinal uses of plants in Gilgit-Baltistan, Pakistan. J Med Plants Res. 2014;8(30):992–1004.View ArticleGoogle Scholar
  77. Khan N, Ahmed M, Ahmed A, Shaukat SS, Wahab M, Ajaib M, Siddiqui FM, Nasir M. Important medicinal plants of Chitral Gol National Park (CGNP) Pakistan. Pak J Bot. 2011;43(2):797–809.Google Scholar
  78. Parvaiz M, Bhatti KH, Nawaz K, Hussain Z, Khan RMW, Hussain A. Ethno-botanical studies of medicinal plants of Dinga, District Gujrat, Punjab, Pakistan. World Appl Sci J. 2013;26(6):826–33.Google Scholar
  79. Husain SZ, Malik RN, Javaid M, Bibi S. Ethonobotanical properties and uses of medicinal plants of Morgah biodiversity park, Rawalpindi. Pak J Bot. 2008;40(5):1897–911.Google Scholar
  80. Awan AA, Murtaza G. Ethnobotanical uses of plants of family Solanaceae muzaffarabad division Azad Jammu and Kashmir, Pakistan-13100. Int Pharm Sci Invention. 2013;2(7):5–11.Google Scholar
  81. Shah A, Marwat KS, Gohar F, Khan A, Bhatti HK, Amin M, Ud-Din N, Ahmad M, Zafar M. Ethnobotanical study of medicinal plants of semi-tribal area of Makerwal & Gulla Khel (lying between Khyber Pakhtunkhwa and Punjab Provinces), Pakistan. Am J Plant Sci. 2013;4:98–116.View ArticleGoogle Scholar
  82. Jan G, Khan MA, Gul F. Ethnomedicinal plants used against diarrhea and dysentery in Dir Kohistan valley (NWFP), Pakistan. Ethnobot Leaflets. 2008;12:620–37.Google Scholar
  83. Khan B, Abdukadir A, Qureshi R, Mustafa G. Medicinal uses of plants by the inhabitants of Khunjerab National Park, Gilgit, Pakistan. Pak J Bot. 2011;43(5):2301–10.Google Scholar
  84. Afzal S, Afzal N, Awan RM, Khan ST, Gilani A, Khanum R, Tariq S. Ethno-botanical studies from Northern Pakistan. J Ayub Med Coll Abbottabad. 2009;21(1):52–7.PubMedPubMed CentralGoogle Scholar
  85. Sarangzai MA, Ahmed A, Laghari KS. Traditional uses of some useful medicinal plants of Ziarat district Balochistan, Pakistan. FUUAST J Biol. 2013;3(1):101–7.Google Scholar
  86. Patil RA, Makwana AB. Anti-hyperbilirubinemic and wound healing activity of aqueous extract of Calotropis procera leaves in Wistar rats. Ind J Pharmacol. 2015;47(4):398.View ArticleGoogle Scholar
  87. Qaiser Joshi AL, Roham PH, Mhaske R, Jadhav M, Krishnadas K, Kharat A, Hardikar B, Kharat KR. Calotropis procera extract induces apoptosis and cell cycle arrest at G2/M phase in human skin melanoma (SK-MEL-2) cells. Nat Prod Res. 2015;29(23):2261–4.View ArticleGoogle Scholar
  88. Aderounmu AO, Omonisi AE, Akingbasote JA, Makanjuola M, Bejide RA, Orafidiya LO, Adelusola KA. Wound-healing and potential anti-keloidal properties of the latex of Calotropis procera (Aiton) Asclepiadaceae in rabbits. Afr J Tradit Complement Altern Med. 2013;10(3):574–9.PubMed CentralPubMedGoogle Scholar
  89. Laitiff AA, Teoh SL, Das S. Wound healing in diabetes mellitus: traditional treatment modalities. Clin Ter. 2010;161(4):359–64.PubMedPubMed CentralGoogle Scholar
  90. Rasik AM, Raghubir R, Gupta A, Shukla A, Dubey PM, Srivastava S, Jain KH, Kulshrestha KD. Healing potential of Calotropis procera on dermal wounds in Guinea pigs. J Ethnopharmacol. 1999;68(1–3):261–6.View ArticlePubMedPubMed CentralGoogle Scholar
  91. Asif A, Kakub G, Mehmood S, Khunum R, Gulfraz M. Wound healing activity of root extracts of Berberis lyceum royle in rats. Phytother Res. 2007;21(6):589–91.View ArticlePubMedPubMed CentralGoogle Scholar
  92. Pirzada AJ, Shaikh W, Usmanghani K, Mohiuddin E. Antifungal activity of Dodonaea viscosa Jacq extract on pathogenic fungi isolated from superficial skin infection. Pak J Pharm Sci. 2010;23(3):337–40.PubMedPubMed CentralGoogle Scholar
  93. Teshome K, Gebre-Mariam T, Asres K, Engidawork E. Toxicity studies on dermal application of plant extract of Dodonaea viscosa used in Ethiopian traditional medicine. Phytother Res. 2010;24(1):60–9.View ArticlePubMedPubMed CentralGoogle Scholar
  94. Patel M, Coogan MM, Teshome K, Gebre-Mariam T, Asres K, Engidawork E. Antifungal activity of the plant Dodonaea viscosa var. angustifolia on Candida albicans from HIV-infected patients. J Ethnopharmacol. 2008;118(1):173–6.View ArticlePubMedPubMed CentralGoogle Scholar
  95. Chakraborty A, Brantner A, Mukainaka T, Nobukuni Y, Kuchide M, Konoshima T, Tokuda H, Nishino H. Cancer chemopreventive activity of Achyranthes aspera leaves on Epstein–Barr virus activation and two-stage mouse skin carcinogenesis. Cancer Lett. 2002;177(1):1–5.View ArticlePubMedPubMed CentralGoogle Scholar
  96. Taye B, Giday M, Animut A, Seid J. Antibacterial activities of selected medicinal plants in traditional treatment of human wounds in Ethiopia. Asian Pac J Trop Biomed. 2011;1(5):370–5.PubMed CentralView ArticlePubMedGoogle Scholar
  97. Barua CC, Talukdar A, Begum SA, Pathak DC, Sarma DK, Borah RS, Gupta A. In vivo wound-healing efficacy and antioxidant activity of Achyranthes aspera in experimental burns. Pharm Biol. 2012;50(7):892–9.View ArticlePubMedPubMed CentralGoogle Scholar
  98. Fikru A, Makonnen E, Eguale T, Debella A, Abie Mekonnen G. Evaluation of in vivo wound healing activity of methanol extract of Achyranthes aspera L. J Ethnopharmacol. 2012;143(2):469–74.View ArticlePubMedPubMed CentralGoogle Scholar
  99. Mukherjee H, Ojha D, Bag P, Chandel HS, Bhattacharyya S, Chatterjee TK, Mukherjee PK, Chakraborti S, Chattopadhyay D. Anti-herpes virus activities of Achyranthes aspera: an indian ethnomedicine, and its triterpene acid. Microbiol Res. 2013;168(4):238–44.View ArticlePubMedPubMed CentralGoogle Scholar
  100. Gangopadhyay KS, Khan M, Pandit S, Chakrabarti S, Mondal TK, Biswas TK. Pharmacological evaluation and chemical standardization of an ayurvedic formulation for wound healing activity. Int J Low Extrem Wounds. 2014;13(1):41–9.View ArticleGoogle Scholar
  101. Benson KF, Newman RA, Jensen GS. Antioxidant, anti-inflammatory, anti-apoptotic, and skin regenerative properties of an Aloe vera-based extract of Nerium oleander leaves (nae-8(®)). Clin Cosmet Investig Dermatol. 2015;8:239–48.PubMed CentralPubMedGoogle Scholar
  102. Ajose FO. Some Nigerian plants of dermatologic importance. Int J Dermatol. 2007;1:48–55.View ArticleGoogle Scholar
  103. Ali-Shtayeh MS, Al-Assali AA, Jamous RM. Antimicrobial activity of Palestinian medicinal plants against acne-inducing bacteria. Univ J Microbiol Res. 2013;7(21):2560–73.Google Scholar
  104. Nemudzivhadi V, Masoko P. In vitro assessment of cytotoxicity, antioxidant, and anti-inflammatory activities of ricinus communis (Euphorbiaceae) leaf extracts. Evid Based Complement Altern Med. 2014;2014:625961.View ArticleGoogle Scholar
  105. Martínez GJ, Barboza GE. Natural pharmacopoeia used in traditional Toba medicine for the treatment of parasitosis and skin disorders (Central Chaco, Argentina). J Ethnopharmacol. 2010;132(1):86–100.View ArticleGoogle Scholar
  106. Lomash V, Parihar SK, Jain NK, Katiyar AK. Effect of Solanum nigrum and Ricinus communis extracts on histamine and carrageenan-induced inflammation in the chicken skin. Cell Mol Biol. 2010;9:56.Google Scholar
  107. Roh JS, Han JY, Kim JH, Hwang JK. Inhibitory effects of active compounds isolated from safflower (Carthamus tinctorius L.) seeds for melanogenesis. BiolPharm Bull. 2004;27(12):1976–8.View ArticleGoogle Scholar
  108. Chen YS, Lee SM, Lin CC, Liu CY, Wu MC, Shi WL. Kinetic study on the tyrosinase and melanin formation inhibitory activities of carthamus yellow isolated from Carthamus tinctorius L. J Biosci Bioeng. 2013;115(3):242–5.View ArticleGoogle Scholar
  109. Kong SZ, Shi XG, Feng XX, Li WJ, Liu WH, Chen ZW, Xie JH, Lai XP, Zhang SX, Zhang XJ, Su ZR. Inhibitory effect of hydroxysafflor yellow on mouse skin photoaging induced by ultraviolet irradiation. Rejuvenation Res. 2013;16(5):404–13.PubMed CentralView ArticlePubMedGoogle Scholar
  110. Joshi A, Sengar N, Prasad SK, Goel RK, Singh A, Hemalatha S. Wound-healing potential of the root extract of Albizzia lebbeck. Planta Med. 2013;79(9):737–43.View ArticlePubMedPubMed CentralGoogle Scholar
  111. Kováč I, Ďurkáč J, Hollý M, Jakubčová K, Peržeľová V, Mučaji P, Smetana K. Plantago lanceolata L. water extract induces transition of fibroblasts into myofibroblasts and increases tensile strength of healing skin wounds. J Pharm Pharmacol. 2015;67(1):117–25.View ArticlePubMedPubMed CentralGoogle Scholar
  112. Sultana S, Khan AM, Ahmad M, Zafar M. Indigenous knowledge of folk herbal medicines by the women of District Chakwal, Pakistan. Ethnobot Leaflets. 2006;10:243–53.Google Scholar
  113. Qureshi R, Bhatti GR, Memon RA. Ethnomedicinal uses of herbs from northern part of Nara desert, Pakistan. Pak J Bot. 2010;42(2):839–51.Google Scholar
  114. Ishtiaq M. Medicinal plant diversity in the flora of Leepa Valley, Muzaffarabad (AJK), Pakistan. Afr J Biotechnol. 2012;11(12):2087–98.Google Scholar
  115. Alam P, Ali M. Phytochemical investigation of Calotropis procera Ait roots. CSIR. 2009;48B(03):443–6.Google Scholar
  116. Sabir S, Tahir K, Rashid N, Naz S, Masood B, Shah MA, Sualeh M. Phytochemical and antioxidant studies of Berberis lycium. Pak J Pharm Sci. 2013;26(6):1165–72.PubMedPubMed CentralGoogle Scholar
  117. Rani MS, Pippalla RS, Mohan K. Dodonaea viscosa Linn.—an overview. Asian J Pharm Res Health Care. 2009;1(1):97–112.Google Scholar
  118. Srivastav S, Singh P, Mishra G, Jha KK, Khosa RL. Achyranthes aspera—n important medicinal plant: a review. J Nat Prod Plant Resour. 2011;1(1):1–14.Google Scholar
  119. Derwich E, Benziane Z, Boukir A. Antibacterial activity and chemical composition of the essential oil from flowers of Nerium oleander. Electronic J Environ Agric Food Chem. 2010;9:6.Google Scholar
  120. Jena J, Gupta AK. Ricinus communis Linn: a phytopharmacological review. Int J Pharm Pharm Sci. 2012;4(4):25–9.Google Scholar
  121. Zhou YZ, Chen H, Qiao L, Hao DF, Hua HM, Pei YH. Chemical constituents from Carthamus tinctorius L. Chinese J Med Chem. 2007;17(6):380.Google Scholar
  122. Zhao Y, Liu F, Lou HX. Studies on the chemical constituents of Solanum nigrum. J Chin Med Mater. 2010;33(4):555–6.Google Scholar
  123. Kokila K, Priyadharshini SD, Sujatha V. Phytopharmacological properties of Albizia species: a review. Int J Pharm Pharm Sci. 2013;5(3):70–3.Google Scholar
  124. Tamura Y, Nishibe S. Changes in the concentrations of bioactive compounds in plantain leaves. J Agric Food Chem. 2002;50(9):2514–8.View ArticlePubMedPubMed CentralGoogle Scholar
  125. Kavishankar GB, Lakshmidevi N, Murthy SM, Prakash HS, Niranjana SR. Diabetes and medicinal plants—a review. Int J Pharm Biomed Sci. 2011;2(3):65–80.Google Scholar
  126. Ahmed N, Mahmood A, Mahmood A, Tahir SS, Bano A, Malik NR, Hassan S, Ishtiaq M. Relative importance of indigenous medicinal plants from Layyah district, Punjab Province, Pakistan. J Ethnopharmacol. 2014;155(1):509–23.View ArticlePubMedPubMed CentralGoogle Scholar
  127. Shinwari KZ, Gilani SS. Sustainable harvest of medicinal plants at Bulashbar Nullah, Astore (Northern Pakistan). J Ethnopharmacol. 2003;84:289–98.View ArticlePubMedPubMed CentralGoogle Scholar
  128. Hussain I, Bano A, Ullah F. Traditional drug therapies from various medicinal plants of Central Karakoram National Park. Pak J Bot. 2011;43:79–84.Google Scholar
  129. Marwat KS, Khan AM, Ahmad M, Zafar M, Ur-Rehman F. Ethnophytomedicines for treatment of various diseases in DI, Khan District. J Agric. 2008;24(2):305–16.Google Scholar
  130. Hamayun M, Khan A, Afzal S, Khan MA. Study on traditional knowledge and utility of medicinal herbs of district Buner, NWFP, Pakistan. Indian J Trad Know. 2006;5(3):407–12.Google Scholar
  131. Ghani A, Batool M. Folk recipes of some medicinal plants used by the inhabitants of soon valley khushab (Pakistan). Int J Curr Pharm Res. 2012;4(1):60–3.Google Scholar
  132. Marwat KS, Ur-Rehman F, Usman K, Khakwani AA, Ghulam S, Anwar N, Sadiq M, Khan JS. Medico-ethnobotanical studies of edible wild fruit plants species from the flora of north western Pakistan (D. I. Khan district). J Med Plants Res. 2011;5(16):3679–86.Google Scholar
  133. Ajaib M, Ud-Din Khan Z. Ethnobotanical studies of useful trees of District Kotli, Azad Jammu and Kashmir. Biologia. 2014;60(1):63–71.Google Scholar
  134. Khan K, Erum A, Ahmad B, Akram M, Anwar M, Junaid A, Saleem U. Ethnobotanical studies from northern areas of Pakistan. Pharmacologyonline. 2009;354:328–54.Google Scholar
  135. Gulshan BA, Dasti AA, Hussain S, Atta IM, Amin-Ud-Din M. Indigenous uses of medicinal plants in rural areas of Dera ghazi khan, Punjab, Pakistan. Agric Biol Sci. 2012;7(9):750.Google Scholar
  136. Hamayun M. Ethnobotanical studies of some useful shrubs and trees of District Buner, NWFP, Pakistan. Ethnobot Leaflets. 2003;1:12.Google Scholar
  137. Pavela R. History, presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects—a review. Plant Prot Sci. 2016;52:229–41.View ArticleGoogle Scholar
  138. Jivad N, Bahmani M, Asadi-Samani M. A review of the most important medicinal plants effective on wound healing on ethnobotany evidence of Iran. Der Pharm Lett. 2016;8(2):353–7.Google Scholar


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